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06-719 Anti-LexA Antibody, DNA-binding region

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06-719
200 µg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
E. coliIP, WBRbPurifiedPolyclonal Antibody
Description
Catalogue Number06-719
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-LexA Antibody, DNA-binding region
Background InformationThe anti-LexA Antibody allows detection of LexA protein and recombinant proteins fused to the LexA protein. This antibody can be used to detect expression of potential bait proteins constructed for use with the yeast two-hybrid system or other interaction trap systems. LexA adds about 22 to 25 kDa to your protein, depending on the number of extra amino acids.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • LexA-pRB transfected yeast cell extract
PresentationProtein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide as a preservative.
Quality LevelMQ100
Applications
ApplicationDetect LexA with Anti-LexA Antibody, DNA-binding region (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Biological Information
ImmunogenHis-tagged DNA binding domain of LexA protein corresponding to amino acid residues 1-202
HostRabbit
SpecificityRecognizes LexA portion of bait fusion proteins used in the yeast two-hybrid system.
IsotypeIgG
Species Reactivity
  • E. coli
Antibody TypePolyclonal Antibody
Entrez Gene Number
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight24 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on LexA-pRB transfected yeast lysate
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
06-719 04053252409004

Documentation

Anti-LexA Antibody, DNA-binding region SDS

Title

Safety Data Sheet (SDS) 

Anti-LexA Antibody, DNA-binding region Certificates of Analysis

TitleLot Number
Anti-LexA, DNA-binding region 3069701
Anti-LexA, DNA-binding region (rabbit polyclonal IgG) - 2121991 2121991
Anti-LexA, DNA-binding region (rabbit polyclonal IgG) - 2462851 2462851
Anti-LexA, DNA-binding region (rabbit polyclonal IgG) 3122939
Anti-LexA, DNA-binding region (rabbit polyclonal IgG) 3027177
Anti-LexA, DNA-binding region (rabbit polyclonal IgG) 2922417
Anti-LexA, DNA-binding region (rabbit polyclonal IgG) - 2201572 2201572
Anti-LexA, DNA-binding region (rabbit polyclonal IgG) - 2377378 2377378
Anti-LexA, DNA-binding region - 16359 16359
Anti-LexA, DNA-binding region - 2018114 2018114

References

Reference overviewApplicationPub Med ID
A signature motif mediating selective interactions of BCL11A with the NR2E/F subfamily of orphan nuclear receptors.
Chan, CM; Fulton, J; Montiel-Duarte, C; Collins, HM; Bharti, N; Wadelin, FR; Moran, PM; Mongan, NP; Heery, DM
Nucleic acids research  41  9663-79  2013

Show Abstract
Western Blotting23975195 23975195
PRAME is a golgi-targeted protein that associates with the Elongin BC complex and is upregulated by interferon-gamma and bacterial PAMPs.
Wadelin, FR; Fulton, J; Collins, HM; Tertipis, N; Bottley, A; Spriggs, KA; Falcone, FH; Heery, DM
PloS one  8  e58052  2013

Show Abstract
Western Blotting23460923 23460923
Notch signaling is antagonized by SAO-1, a novel GYF-domain protein that interacts with the E3 ubiquitin ligase SEL-10 in Caenorhabditis elegans.
Hale, VA; Guiney, EL; Goldberg, LY; Haduong, JH; Kwartler, CS; Scangos, KW; Goutte, C
Genetics  190  1043-57  2012

Show Abstract
22209900 22209900
DNA replication origin function is promoted by H3K4 di-methylation in Saccharomyces cerevisiae.
Rizzardi, LF; Dorn, ES; Strahl, BD; Cook, JG
Genetics  192  371-84  2012

Show Abstract
22851644 22851644
Suppression and recovery of BRCA1-mediated transcription by HP1γ via modulation of promoter occupancy.
Choi, JD; Park, MA; Lee, JS
Nucleic acids research  40  11321-38  2012

Show Abstract
23074186 23074186
Direct identification of insulator components by insertional chromatin immunoprecipitation.
Fujita, T; Fujii, H
PloS one  6  e26109  2011

Show Abstract Full Text Article
22043306 22043306
Insertional chromatin immunoprecipitation: a method for isolating specific genomic regions.
Hoshino, A; Fujii, H
J Biosci Bioeng  108  446-9  2009

Show Abstract
19804873 19804873
Lack of nuclear translocation of cytoplasmic domains of IL-2/IL-15 receptor subunits.
Hodaka Fujii,Akemi Hoshino
Cytokine  46  2009

Show Abstract Full Text Article
19329337 19329337
Genome-wide analysis of the functions of a conserved surface on the corepressor Tup1.
Green, SR; Johnson, AD
Molecular biology of the cell  16  2605-13  2005

Show Abstract
15788561 15788561
Tyrosine residues direct the ubiquitination and degradation of the NY-1 hantavirus G1 cytoplasmic tail.
Erika Geimonen, Imelyn Fernandez, Irina N Gavrilovskaya, Erich R Mackow
Journal of virology  77  10760-868  2003

Show Abstract Full Text Article
14512526 14512526