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05-1154 Sigma-AldrichAnti-JAK1 Antibody, clone 73

Anti-JAK1 Antibody, clone 73 detects level of JAK1 & has been published & validated for use in WB, IP & IF.

Anti-JAK1 Antibody, clone 73 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
Brochures
Technical Information

Research Category: Signaling Research Sub-Category: Immunological Signaling Gene Symbol: JAK1, JAK1B, JAK-1, JAK1A, EC 2.7.10.2 UniProt Number: P23458

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Overview

Replacement Information
Replacement Information	05-1154 is a recommended replacement for MAB3700

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R, Ca, Xn, Ch	WB, IP, IF	M	Purified	Monoclonal Antibody

Description
Catalogue Number	05-1154
Replaces	MAB3700
Description	Anti-JAK1 Antibody, clone 73
Alternate Names	Janus kinase 1 Janus kinase 1 (a protein tyrosine kinase) 1 Tyrosine-protein kinase JAK1
Background Information	JAKs are non-receptor tyrosine kinases which couple to cytokine receptors, and are activated by cytokines. They regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. JAK kinases (JAK1, JAK2, JAK3, Tyk2) have a kinase domain and a second, pseudokinase domain which is not enzymatically active. The principal effectors of JAKs are the STATs (Signal Transducers and Activators of Transcription), which are direct activators of transcription, but JAKs also signal to the Ras-MAP Kinase pathway, and through Tec, to the PI3 Kinase pathway. Immunological stimuli, such as interferons and cytokines, induce recruitment of STAT transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal STAT transcription factors, which subsequently dimerize and translocate to the nucleus where they bind DNA. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor. Among a variety of sites, Tyrosines 1007 and 1008 (Tyr1007/1008) are sites of trans- or autophosphorylation in vivo and in vitro kinase reactions. JAK2 is required for signaling through a different subset of cytokine receptors, while JAK1 is essential for signaling from the IL-2, IL-6 and interferon receptors. JAK3 is required for signaling through cytokine receptors which share a common gamma-chain.

References

Product Information
Format	Purified
Control	Huvec cell lysate, whole Jurkat cell lysate
Presentation	Purified mouse monoclonal IgG2b aqueous buffered solution containing 50% glycerol, BSA, and < 0.09% sodium azide
Quality Level	MQ100

Applications
Application	Anti-JAK1 Antibody, clone 73 detects level of JAK1 & has been published & validated for use in WB, IP & IF.
Key Applications	Western Blotting Immunoprecipitation Immunofluorescence
Application Notes	Immunoprecipitation: A previous lot of this antibody was used in immunoprecipitation under native and denatured conditions. Immunofluorescence: A previous lot of this antibody was used in IF. Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Generated from human JAK1, amino acids 551-776.
Clone	73
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Recognizes JAK1 (Janus Kinase 1). The members of the JAK family of cytoplasmic protein tyrosine kinases physically associate with ligand-bound receptors. It is this association that results in tyrosine phosphorylation and activation. JAK1 is approximately 130 kDa and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among JAK family members.
Isotype	IgG2b
Species Reactivity	Human Mouse Rat Canine Xenopus Chicken
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_002227.2
Entrez Gene Summary	Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.
Gene Symbol	JAK1 JAK1B JAK-1 JAK1A EC 2.7.10.2
Purification Method	Purified
UniProt Number	P23458
UniProt Summary	FUNCTION: SwissProt: P23458 # Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor. SIZE: 1142 amino acids; 131957 Da SUBUNIT: Interacts with IL31RA and SHB. SUBCELLULAR LOCATION: Intracytoplasmic membrane; Peripheral membrane protein. Note=Wholly intracellular, possibly membrane associated. DOMAIN: SwissProt: P23458 Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1. SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain. & Contains 1 SH2 domain.
Molecular Weight	130 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on Huvec lysates. Western Blot Analysis: 1:500 dilution of this lot detected JAK1 on 10 μg of Huvec lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable frozen at -20°C in undiluted aliquots for up to 1 year from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable frozen at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
05-1154	04053252735509

Documentation

Anti-JAK1 Antibody, clone 73 SDS

Title
Safety Data Sheet (SDS)

Anti-JAK1 Antibody, clone 73 Certificates of Analysis

Title	Lot Number
Anti-JAK1, clone 73	3035205
Anti-JAK1, clone 73 - 2426532	2426532
Anti-JAK1, clone 73 - 2204996	2204996
Anti-JAK1, clone 73 - 2283924	2283924
Anti-JAK1, clone 73 - 3552763	3552763
Anti-JAK1, clone 73 - 3611144	3611144
Anti-JAK1, clone 73 - 3720639	3720639
Anti-JAK1, clone 73 - 3918082	3918082
Anti-JAK1, clone 73 - NG1584118	NG1584118
Anti-JAK1, clone 73 -2500435	2500435

Brochure

Title
Pathways and Biomarkers of JAK/STAT Signaling

Technical Info

Title
JAK/STAT Signaling Research Focus

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