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407143
Sigma-AldrichAnti-IP₃ Receptor Rabbit pAb
This Anti-IP₃ Receptor Rabbit pAb is validated for use in ELISA, FC, Immunoblotting, IF, IHC, IP, Radioimmunoassay for the detection of IP₃ Receptor.
More>>This Anti-IP₃ Receptor Rabbit pAb is validated for use in ELISA, FC, Immunoblotting, IF, IHC, IP, Radioimmunoassay for the detection of IP₃ Receptor. Less<<
Anti-IP₃ Receptor Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Variables associated with assay conditions will dictate the proper working dilution.
Recommended Protocol for Immunoblotting using anti-IP3 Receptor Antibodies
NOTE: For protein loading, we recommend 1 x 106 cells/gel lane or 100 μg/gel lane.
• TBST • 20 mM Tris-HCl, pH 7.6 • 150 mM NaCl • 0.05% Tween®-20 detergent
1. After transfer, block in 15 ml of TBST with 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody). 2. Dilute primary antibody in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST. 3. Incubate with polyclonal antibody for 1 h or with monoclonal antibody for 2 h. 4. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST. 5. Dilute secondary antibody also in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST, followed by HRP. 6. Shake for 1 h. 7. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST. 8. Mix development solution (1:1) (Amersham ECL kit). 9. In between two pieces of transparency add development solution. Allow to sit for approximately 1 min before exposure to film.
Biological Information
Immunogen
a synthetic peptide corresponding to amino acids in the C-terminal cytoplasmic domain of the IP₃ receptor
Host
Rabbit
Isotype
IgG
Species Reactivity
Bovine
Canine
Human
Mouse
Porcine
Rat
Antibody Type
Polyclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Anti-IP₃ Receptor Rabbit pAb Certificates of Analysis
Title
Lot Number
407143
References
Reference overview
Bourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29. Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290. Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Rabbit polyclonal antibody purified by ammonium sulfate precipitation and ion-exchange (DEAE) column chromatography. Recognizes the ~260 kDa IP3 receptor protein.
Background
Inositol 1,4,5-triphosphate (IP3) receptors have been shown to play an important role in the regulation of Ca2+ release from internal storage sites during cell activation.
Host
Rabbit
Immunogen
a synthetic peptide corresponding to amino acids in the C-terminal cytoplasmic domain of the IP₃ receptor
Isotype
IgG
Species
bovine, canine, human, mouse, porcine, rat
Form
Liquid
Formulation
In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
Concentration Label
Please refer to vial label for lot-specific concentration
Preservative
≤0.1% sodium azide
Comments
Variables associated with assay conditions will dictate the proper working dilution.
Recommended Protocol for Immunoblotting using anti-IP3 Receptor Antibodies
NOTE: For protein loading, we recommend 1 x 106 cells/gel lane or 100 μg/gel lane.
• TBST • 20 mM Tris-HCl, pH 7.6 • 150 mM NaCl • 0.05% Tween®-20 detergent
1. After transfer, block in 15 ml of TBST with 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody). 2. Dilute primary antibody in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST. 3. Incubate with polyclonal antibody for 1 h or with monoclonal antibody for 2 h. 4. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST. 5. Dilute secondary antibody also in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST, followed by HRP. 6. Shake for 1 h. 7. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST. 8. Mix development solution (1:1) (Amersham ECL kit). 9. In between two pieces of transparency add development solution. Allow to sit for approximately 1 min before exposure to film.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Standard Handling
References
Bourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29. Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290. Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.