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05-1571 Anti-IGF-1R Antibody, clone 24-57

This Anti-IGF-1R Antibody, clone 24-57 is validated for use in IH, IP for the detection of IGF-1R.

Anti-IGF-1R Antibody, clone 24-57 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Metabolism Research Sub-Category: Insulin/Energy Signaling Gene Symbol: IGF1R, CD221, IGFIR, IGFR, JTK13 UniProt Number: P08069
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05-1571
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIHC, IPMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1571
DescriptionAnti-IGF-1R Antibody, clone 24-57
Alternate Names
  • CD221 antigen
  • IGF-I receptor
  • Insulin-like growth factor I receptor
  • insulin-like growth factor 1 receptor
  • soluble IGF1R variant 1
  • soluble IGF1R variant 2
Background InformationThere are two kinds of IGF receptors based on their relative affinities for IGF1 versus IGF2. The IGF1 receptor prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kDa) and two beta (95 kDa) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.
References
Product Information
FormatPurified
Control
  • Human placental villi and tonsil tissue
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-IGF-1R Antibody, clone 24-57 is validated for use in IH, IP for the detection of IGF-1R.
Key Applications
  • Immunohistochemistry
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Soos, M., et al. (1992). Journal of Biological Chemistry. 267(18):12955-12963.)
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human IGF-IR.
EpitopeUnknown
Clone24-57
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes IGF-IR.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq].
Gene Symbol
  • IGF1R
  • CD221
  • IGFIR
  • IGFR
  • JTK13
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade.

SIZE: 1367 amino acids; 154793 Da

SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

TISSUE SPECIFICITY: Expressed in a variety of tissues.

PTM: The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin- like growth factor I (IGF I). & Phosphorylation of Tyr-980 is required for IRS1- and SHC1- binding.

DISEASE: Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM:270450]. IGF1 resistance is a growth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1.

SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 3 fibronectin type-III domains. & Contains 1 protein kinase domain.
Molecular Weight155 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human placental villi and tonsil tissue.

Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected IGF-1R in human placental villi and tonsil tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-1571 04053252363559

Documentation

Anti-IGF-1R Antibody, clone 24-57 SDS

Title

Safety Data Sheet (SDS) 

Anti-IGF-1R Antibody, clone 24-57 Certificates of Analysis

TitleLot Number
Anti-IGF-1R, clone 24-57 2485684
Anti-IGF-1R, clone 24-57 - 2410231 2410231
Anti-IGF-1R, clone 24-57 - 2066717 2066717
Anti-IGF-1R, clone 24-57 - 2205005 2205005
Anti-IGF-1R, clone 24-57 - 3025881 3025881
Anti-IGF-1R, clone 24-57 - 3232421 3232421
Anti-IGF-1R, clone 24-57 - 3323028 3323028
Anti-IGF-1R, clone 24-57 - NG1838620 NG1838620
Anti-IGF-1R, clone 24-57 - NRG1697045 NRG1697045
Anti-IGF-1R, clone 24-57 -2547204 2547204
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