Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MAB2146Z Anti-ICAM-1 Antibody, clone P2A4, Azide Free

View Products on Sigmaaldrich.com
MAB2146Z
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, FC, IP, ICC, IHC, FUNCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB2146Z
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-ICAM-1 Antibody, clone P2A4, Azide Free
Alternate Names
  • CD54
  • MAB2146
Product Information
FormatPurified
PresentationSterile-filtered lquid containing 0.2M phosphate buffer, 0.25M sodium chloride, no preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-ICAM-1 Antibody, clone P2A4, Azide Free is validated for use in ELISA, FC, IP, IC, IH, FUNC for the detection of ICAM-1.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunoprecipitation
  • Immunocytochemistry
  • Immunohistochemistry
  • Affects Function
Application NotesImmunohistochemistry: for use on acetone or paraformaldehyde fixed tissue

Immunocytochemistry: for use on B cells, PBL, T cells

Immunoprecipitation

FACS

ELISA

Inhibits ICAM-1 dependent adhesive interactions

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenIL-1beta activated HUVEC (human)
CloneP2A4
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityHuman ICAM-1
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryICAM1 (CD54) is typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. ICAM1 is also exploited by Rhinovirus as a receptor.
Gene Symbol
  • ICAM1
  • P3.58
  • BB2
  • CD54
  • ICAM-1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P05362 # ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). In case of rhinovirus infection acts as a cellular receptor for the virus. Also acts as receptor for some coxsackieviruses.
SIZE: 532 amino acids; 57825 Da
SUBUNIT: Interacts with human herpesvirus 8 MIR2 protein (Probable).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
PTM: Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
SIMILARITY: SwissProt: P05362 ## Belongs to the immunoglobulin superfamily. ICAM family. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MAB2146Z 04053252661990