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ABE327 Anti-Histone H2A Antibody

Use Anti-Histone H2A Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect Histone H2A also known as Histone H2A type 1, H2A.1, Histone H2A/p.

Anti-Histone H2A Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H2AG, H2AFP, HIST1H2AI, H2AFC, HIST1H2AK, H2AFD, HIST1H2AL, H2AFI, HIST1H2AM, H2AFN UniProt Number: P0C0S8
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ABE327
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE327
DescriptionAnti-Histone H2A Antibody
Alternate Names
  • Histone H2A type 1
  • H2A.1
  • Histone H2A/p
Background InformationHistone H2A is one of four components of the core nucleosomal structure. The nucleosome represents a unit of chromatin in which DNA is wrapped around a histone octamer. Histones undergo a number of post-translational modifications (PTM) in response to various stimuli that may induce changes in the structure of the nucleosome and hide or expose DNA sequences. Histone H2A, in particular, undergoes acetylation on lysine 5 by the Tip60 enzyme which may promote unfolding of chromatin and transcription. In addition, H2A is phosphorylated on serine 1 and serine 139 (H2A.X) residues which mark mitosis and gene repression, and DNA damage, respectively. Gene repression may also result from the sumoylation of H2A’s lysine 126 residue. In addition, ubiquitination of the H2A lysine 119 residue may play a role in spermatogenesis. It is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.
References
Product Information
FormatAffinity Purified
Control
  • HeLa acid extract and recombinant histone proteins
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-Histone H2A Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect Histone H2A also known as Histone H2A type 1, H2A.1, Histone H2A/p.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistchemistry Analysis: A 1:1,000 dilution from a representative lot detected Histone H2A in liver and kidney tissues.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human Histone H2A.
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes Histone H2A at the C-terminus.
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteDemonstrated to react with Human, Mouse, and Rat. Broad species reactivity expected based on sequence similarity.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H2AG
  • H2AFP
  • HIST1H2AI
  • H2AFC
  • HIST1H2AK
  • H2AFD
  • HIST1H2AL
  • H2AFI
  • HIST1H2AM
  • H2AFN
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~16 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa acid extract and recombinant histone proteins.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Histone H2A on 10 µg of HeLa acid extract and recombinant histone proteins.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE327 04053252691553

Documentation

Anti-Histone H2A Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Histone H2A Antibody Certificates of Analysis

TitleLot Number
Anti-Histone H2A - 2388321 2388321
Anti-Histone H2A - 2420535 2420535
Anti-Histone H2A - 2461570 2461570
Anti-Histone H2A - 2197286 2197286
Anti-Histone H2A - 2276377 2276377
Anti-Histone H2A - 2363605 2363605
Anti-Histone H2A - 3229379 3229379
Anti-Histone H2A - 3398045 3398045
Anti-Histone H2A - 3527812 3527812
Anti-Histone H2A - 3715716 3715716
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Brochure

Title
New Products: Volume 3, 2012