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MAB8672-I Anti-HSV1-ICP8 Antibody, clone 10A3

This mouse monoclonal Anti-HSV1-ICP8, clone 10A3 , Cat. No. MAB8672-I is validated for use in Western Blotting for the detection of Herpes Simplex Virus Infected cell protein 8.

Anti-HSV1-ICP8 Antibody, clone 10A3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: DBP, ICP8, UL29 UniProt Number: P04296
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MAB8672-I
100 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB8672-I
DescriptionAnti-HSV1-ICP8 Antibody, clone 10A3
Alternate Names
  • Major DNA-binding protein
  • HSV1-ICP8
  • Infected cell protein 8
Background InformationMajor DNA-binding protein (UniProt: P04296) is encoded by DBP (also known as Infected cell protein 8, ICP8, UL29) gene (Gene ID: 2703458) in human herpes simplex virus 1. HSV1-ICP8 is a 128 kDa, single stranded DNA binding protein of the herpesviridae family. It is an essential replication factor for both Herpes Simplex Virus Type 1 (HSV-1) and Adeno-Associated Virus (AAV) DNA replication. It binds tightly and cooperatively to ssDNA and destabilizes dsDNA helices, promotes DNA strand transfer, and renaturation of ssDNA. ICP8 is reported to regulate viral gene expression by repressing transcription from the parental viral genome and stimulating gene expression from the progeny genome. It is known to associate in replication compartments with over 50 cellular and viral proteins-many of which are involved in DNA replication and repair, recombination, and chromatin modeling. In the absence of DNA replication, HSV1-ICP8 is found in the nuclear framework-associated structures (pre-replicative sites); however, as viral DNA replication proceeds, it migrates to globular intranuclear structures (replication compartments). (Ref.: Glauser, D.L., et al. (2007). J. Virol. 81(9): 4732-4743; Tolun, G., et al. (2013). Nucleic Acids Res. 41(11): 5927 5937).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal Anti-HSV1-ICP8, clone 10A3 , Cat. No. MAB8672-I is validated for use in Western Blotting for the detection of Herpes Simplex Virus Infected cell protein 8.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected HSV1-ICP8 in HeLa cells (Glauser, D.L., et. al. (2007). J Virol. 81(9):4732-43).
Biological Information
ImmunogenICP8 purified from U-35-VERO cells.
Clone10A3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 10A3 detects ICP-8 protein in human herpes simplex virus 1.
IsotypeIgG1κ
Species Reactivity
  • All
Species Reactivity NoteVirus.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • DBP
  • ICP8
  • UL29
Purification MethodProtein G purified
UniProt Number
Molecular Weight~128 kDa observed; 128.35 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in using recombinant protein.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected 1.25 µg of 1CB8 recombinant protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB8672-I 04054839153136