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PC408 Anti-Green Fluorescent Protein (26-39) Rabbit pAb

Anti-Green Fluorescent Protein (26-39), rabbit polyclonal, recognizes the ~28 kDa GFP and some GFP fusion proteins. It is validated for use in Western blotting and immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-GFP

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PC408
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Overview

Replacement Information

Key Spec Table

HostAntibody Type
RbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
PC408-100ULCN Plastic ampoule 100 ul
Description
OverviewRecognizes the ~28 kDa GFP and some GFP fusion proteins.
Catalogue NumberPC408
Brand Family Calbiochem®
SynonymsAnti-GFP
References
ReferencesCubitt, A.B., et al. 1995. Trends Biochem. Sci. 20, 448.
Product Information
FormLiquid
FormulationIn PBS, 1 mg/ml BSA.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesImmunoblotting (1:2000)
Immunocytochemistry (1:200)
Application CommentsThis sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. This antibody has been successfully used in immunoblotting and immunocytochemistry protocols to detect GFP and fusion proteins. It detects a ~28 kDa protein that corresponds to GFP in immunoblots of protein extract from cells transfected with, and overexpressing, GFP isoforms. Antibody should be titrated for optimal results in individual sytems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 26-39 of Aequorea victoria wild-type green fluorescent protein
ImmunogenAequora victoria
HostRabbit
IsotypeIgG
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC408-100ULCN 04055977224573

Documentation

Anti-Green Fluorescent Protein (26-39) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Green Fluorescent Protein (26-39) Rabbit pAb Certificates of Analysis

TitleLot Number
PC408

References

Reference overview
Cubitt, A.B., et al. 1995. Trends Biochem. Sci. 20, 448.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-October-2007 RFH
SynonymsAnti-GFP
ApplicationImmunoblotting (1:2000)
Immunocytochemistry (1:200)
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa GFP and some GFP fusion proteins.
BackgroundGreen Fluorescent Protein (GFP) is responsible for bioluminescence in the jellyfish Aequorea victoria. It has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
HostRabbit
Immunogen speciesAequora victoria
Immunogena synthetic peptide corresponding to amino acids 26-39 of Aequorea victoria wild-type green fluorescent protein
IsotypeIgG
FormLiquid
FormulationIn PBS, 1 mg/ml BSA.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. This antibody has been successfully used in immunoblotting and immunocytochemistry protocols to detect GFP and fusion proteins. It detects a ~28 kDa protein that corresponds to GFP in immunoblots of protein extract from cells transfected with, and overexpressing, GFP isoforms. Antibody should be titrated for optimal results in individual sytems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesCubitt, A.B., et al. 1995. Trends Biochem. Sci. 20, 448.