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06-579-I-25UG Anti-Gab1 Antibody, CT

Anti-Gab1, CT, Cat. No. 06-579-I, is a rabbit polyclonal antibody that detects GRB2-associated-binding protein 1 (GAB1) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

06-579-I-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number06-579-I-25UG
DescriptionAnti-Gab1 Antibody, CT
Alternate Names
  • GRB2-associated-binding protein 1
  • GRB2-associated binder 1
  • Growth factor receptor bound protein 2-associated protein 1
Background InformationGRB2-associated-binding protein 1 (UniProt: Q13480; also known as GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1, Gab1) is encoded by the GAB1 gene (Gene ID: 2549) in human. Gab1 is a signaling adaptor protein that acts as a platform for intracellular signal transduction triggered by activated receptor-type kinases and is involved in growth, transformation, and apoptosis. It plays a role in FGFR1, EGFR, and insulin receptor signaling and is shown to be phosphorylated on tyrosine residues in response to FGFR1, EGFR, and insulin receptor activation. These phosphorylations allow it to interact with several proteins that contain SH2 domains. Some of these phosphorylation sites serve as binding sites for phosphoatidylinositol 3 kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylations of Gab1 on tyrosine 627 and 659 are shown to be is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF. Gab1 is also reported to be a critical component in the interaction between focal adhesion kinase and Met in cancer cell invasion. Mutations in GAB1 gene have been linked to autosomal recessive deafness that is characterized by prelingual, severe to profound hearing loss. (Ref.: Mouradian, M., et al. (2014). Prostaglandins Leukot Essent Fatty Acids. 2014 Apr; 90(4);105-115).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Gab1, CT, Cat. No. 06-579-I, is a rabbit polyclonal antibody that detects GRB2-associated-binding protein 1 (GAB1) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Gab1 in human small intestine and human breast cancer tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 31 amino acids from the C-terminal region of human GRB2-associated-binding protein 1 (Gab1).
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects GRB2-associated-binding protein 1 (Gab1). It targets an epitope within 31 amino acids from the C-terminal region.
IsotypeIgG
Species Reactivity
  • Human
  • Bovine
Species Reactivity NoteHuman, Bovine. Predicted to react with Mouse, Hamster, Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • GAB1
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~100 kDa observed; 76.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Bovine Aorta tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GRB2-associated-binding protein 1 (Gab1) in Bovine aorta tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
06-579-I-25UG 04061841099079