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CB1001
Sigma-AldrichAnti-GAPDH Mouse mAb (6C5)
Anti-GAPDH, mouse monoclonal, clone 6C5, recognizes ~146 kDa GAPDH protein under non-reducing conditions & 36 kDa monomers under reducing conditions. It is validated for use in ELISA, WB, and IHC.
More>>Anti-GAPDH, mouse monoclonal, clone 6C5, recognizes ~146 kDa GAPDH protein under non-reducing conditions & 36 kDa monomers under reducing conditions. It is validated for use in ELISA, WB, and IHC. Less<<
Anti-GAPDH Mouse mAb (6C5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ELISA (see comments) Immunoblotting (1-10 µg/ml; see application references) Immunocytochemistry (see comments)
Application Comments
Useful as a loading or positive control since GAPDH is expressed in all cells. Also reported to work for ELISA and immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
purified, rabbit muscle
Immunogen
Rabbit
Clone
6C5
Host
Mouse
Isotype
IgG₁
Species Reactivity
Canine
Chicken
Fish
Frog
Human
Mouse
Porcine
Rabbit
Rat
Antibody Type
Monoclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Highly Toxic
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Anti-GAPDH Mouse mAb (6C5) Certificates of Analysis
Title
Lot Number
CB1001
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
30-November-2007 JSW
Synonyms
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase
Application
ELISA (see comments) Immunoblotting (1-10 µg/ml; see application references) Immunocytochemistry (see comments)
Application Data
Detection of rat GAPDH by immunocytochemistry. Sample: A-10 cell line (rat aortic smooth muscle cells). Primary antibody: Anti-GAPDH Mouse mAb (6C5) (Cat. No. CB1001) (assay dependent of Primary Antibody). Detection: fluorescence. Detection of GAPDH by immunoblotting. Samples: Tissue exctract from isolated human GAPDH (lane 1), human heart (lane 2), pig heart (lane 3), goat heart (lane 4), bovine heart (lane 5), dog heart (lane 6), mouse heart (lane 7), rat heart (lane 8), rabbit heart (lane 9), and duck heart (lane 10). Primary antibody: Anti-GAPDH Mouse mAb (6C5) (Cat. No. CB1001) (1 µg/ml). Detection: chemiluminescence.
Description
Protein A purified mouse monoclonal antibody. Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.
Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key enzymes involved in glycolysis. GAPDH is comprised of four ~36 kDa subunits with a total molecular weight of ~146 kDa. Besides functioning as a glycolytic enzyme in cytoplasm, mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recently, findings have been made concerning the role of GAPDH in differentpathologies including prostate cancer progression, programmed neuronal cell death, and age-related neuronal diseases such as Alzheimer’s Disease and Huntington’s Disease. While GAPDH is constitutively expressed in almost all tissues at high levels, there are some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types.
Host
Mouse
Immunogen species
Rabbit
Immunogen
purified, rabbit muscle
Clone
6C5
Isotype
IgG₁
Species
not bovine, canine, chicken, fish, frog, human, mouse, porcine, rabbit, rat, not yeast
Positive control
Rat skeletal muscle
Form
Liquid
Formulation
In PBS, pH 7.4.
Concentration Label
Please refer to vial label for lot-specific concentration
Preservative
≤0.1% sodium azide
Comments
Useful as a loading or positive control since GAPDH is expressed in all cells. Also reported to work for ELISA and immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Toxicity
Highly Toxic
Application references
Immunoblotting
Gagarin, D., et al. 2005. J. Mol. Cell. Card.39, 453.
Yang, Z., et al. 2007. J. Vasc. Res.44, 483.
