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MAB1072 Anti-Erythropoietin Antibody, clone 1C8

Detect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB.

Anti-Erythropoietin Antibody, clone 1C8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Metabolism Research Sub-Category: Metabolic Hormones & Receptors Gene Symbol: EPO, MGC138142, EP UniProt Number: P01588
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MAB1072
250 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, IF, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB1072
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Erythropoietin Antibody, clone 1C8
Alternate Names
  • erythropoietin
  • Epoetin
  • epoetin
Background InformationErythropoietin (UniProt: P01588; also known as Epoetin, EPO) is encoded the EPO gene (Gene ID: 2056) in human. EPO is an acidic glycoprotein that is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved off in the endoplasmic reticulum and the mature protein undergoes glycosylation in the Golgi prior to its release. EPO is mainly produced by peritubular fibroblasts in the renal cortex and to some extent by the liver in adult mammals. In fetal and neonatal mammals, it produced in the liver. EPO is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia. It is induced by ischemic preconditioning by activation of (HIF)-1alpha. It is reported to contribute to angiogenesis in response to ischemia through upregulation of VEGF/VEGFR. It binds to EPO receptor that leads to receptor dimerization and JAK2 activation that results in activation of specific downstream effectors, including STAT1 and STAT3. Endogenous EPO consists of three N-linked polysaccharide groups and one O-linked group. Although its glycosylation slightly reduces its biological activity but is shown to be essential for ensuring its prolonged circulation in the plasma. The N-glycans in its structure protect it from the action of proteases and in the modulation of its receptor binding affinity. Mutations in EPO gene can cause microvascular complications in type II diabetic subjects and erythrocytosis. (Ref.: Bunn, HF (2013). Cold Spring Harb. Perspect. Med. 3(3); a011619).
References
Product Information
FormatPurified
Control
  • Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
PresentationPurified mouse monoclonal IgG2b in buffer containing 20 mM sodium phosphate, 300 mM NaCl, and 0.02% sodium merthiolate.
Quality LevelMQ100
Applications
ApplicationDetect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB.
Key Applications
  • ELISA
  • Immunofluorescence
  • Western Blotting
Application NotesELISA:
For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate.

Immunosorbent preparation

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenRecombinant human Epo.
Clone1C8
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with human Erythropoietin (Epo).
IsotypeIgG2b
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
Gene Symbol
  • EPO
  • MGC138142
  • EP
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01588 # Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
SIZE: 193 amino acids; 21307 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
SIMILARITY: SwissProt: P01588 ## Belongs to the EPO/TPO family.
Molecular Weight34 kDa (glycosylated)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot on Mouse kidney lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size250 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB1072 04053252266386

Documentation

Anti-Erythropoietin Antibody, clone 1C8 SDS

Title

Safety Data Sheet (SDS) 

Anti-Erythropoietin Antibody, clone 1C8 Certificates of Analysis

TitleLot Number
Anti-Erythropoietin, clone 1C8 - 2121751 2121751
Anti-Erythropoietin, clone 1C8 - 2148240 2148240
Anti-Erythropoietin, clone 1C8 - 2111385 2111385
Anti-Erythropoietin, clone 1C8 - 2197223 2197223
Anti-Erythropoietin, clone 1C8 - 2327716 2327716
Anti-Erythropoietin, clone 1C8 - 2330589 2330589
Anti-Erythropoietin, clone 1C8 - 3184765 3184765
Anti-Erythropoietin, clone 1C8 - 3207103 3207103
Anti-Erythropoietin, clone 1C8 - 4082572 4082572
Anti-Erythropoietin, clone 1C8 - LV1514199 LV1514199
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