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CP18
Sigma-AldrichAnti-Collagen Type II (Ab-1) Mouse mAb (II-4C11)
This Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11) is validated for use in Frozen Sections for the detection of Collagen Type II (Ab-1).
More>>This Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11) is validated for use in Frozen Sections for the detection of Collagen Type II (Ab-1). Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Recognizes the ~130 kDa type II collagen protein. May react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected.
Catalogue Number
CP18
Brand Family
Calbiochem®
References
References
Kumagai, J. et al. 1994 J. Anat.185, 278. Kivrikko, K,I. 1993 Ann. Med.25, 113. Sandell, L.J., et al. 1991 J. Cell. Biol.114, 1307. Ryan, M.C., and Sandell, L.J. 1990 J. Biol. Chem.265, 10334. Mayne, R., and Burgeson, R.E. 1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL
Product Information
Declaration
Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
Form
Liquid
Formulation
In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Frozen Sections (1-10 µg/ml) Immunoblotting (1-5 µg/ml, see application references) Paraffin Sections (1-10 µg/ml, see application references)
Application Comments
May react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected. This antibody may also react with other classes of collagen at a concentration higher than 1 µg/ml. In immunoblotting, a major band of ~130 kDa is obtained. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
purified collagen type II
Immunogen
Human
Clone
II-4C11
Host
Mouse
Isotype
IgG₁
Species Reactivity
Bovine
Human
Rabbit
Rat
Antibody Type
Monoclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number
GTIN
CP18-100UGCN
04055977220957
Documentation
Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11) SDS
Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11) Certificates of Analysis
Title
Lot Number
CP18
References
Reference overview
Kumagai, J. et al. 1994 J. Anat.185, 278. Kivrikko, K,I. 1993 Ann. Med.25, 113. Sandell, L.J., et al. 1991 J. Cell. Biol.114, 1307. Ryan, M.C., and Sandell, L.J. 1990 J. Biol. Chem.265, 10334. Mayne, R., and Burgeson, R.E. 1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
10-July-2007 JSW
Application
Frozen Sections (1-10 µg/ml) Immunoblotting (1-5 µg/ml, see application references) Paraffin Sections (1-10 µg/ml, see application references)
Description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-X-63-Ag8-LUI mouse myeloma cells. Recognizes the ~130 kDa type II collagen protein.
Background
Collagens are the major fibrous structural elements of cartilage, tendon, skin, bone, lung and blood vessels. Their function is to hold cells together and thereby form discrete tissues. Collagens form insoluble fibers that have a high intrinsic tensile strength. Structurally, the collagens are composed of three polypeptide chains, αchains, which exist as three distinct subclasses each of which in turn is made up of numerous subtypes. Type II collagen is a major component of cartilage. It is translated as a pro-form which is then processed to yield the mature protein. Collagen II can also be expressed in two different forms, IIA and IIB, as a result of alternate splicing involving the inclusion of exon. The protein is synthesized primarily by chondrocytes and by certain cells in the eye, and exists as a trimer of α1(II) chains. Mutations in collagen II have been documented in chondrodysplasias and ostoarthrosis.
Host
Mouse
Immunogen species
Human
Immunogen
purified collagen type II
Clone
II-4C11
Isotype
IgG₁
Species
bovine, human, rabbit, rat
Form
Liquid
Formulation
In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Concentration Label
Please refer to vial label for lot-specific concentration
Preservative
≤0.1% sodium azide
Comments
May react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected. This antibody may also react with other classes of collagen at a concentration higher than 1 µg/ml. In immunoblotting, a major band of ~130 kDa is obtained. Antibody should be titrated for optimal results in individual systems.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Standard Handling
References
Kumagai, J. et al. 1994 J. Anat.185, 278. Kivrikko, K,I. 1993 Ann. Med.25, 113. Sandell, L.J., et al. 1991 J. Cell. Biol.114, 1307. Ryan, M.C., and Sandell, L.J. 1990 J. Biol. Chem.265, 10334. Mayne, R., and Burgeson, R.E. 1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL
Application references
Immunoblotting
Kumagai, J. et al. 1994 J. Anat.185, 278.
Paraffin Sections
Kumagai, J. et al. 1994 J. Anat.185, 278.
