Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

AB2301-25UL Anti-Calnexin (rabbit polyclonal)

Anti-Calnexin Antibody is an antibody against Calnexin for use in WB, IC, IH(P).

Anti-Calnexin (rabbit polyclonal) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: CANX UniProt Number: P27824
View Products on Sigmaaldrich.com
AB2301-25UL
25 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, Chp, Rhesus Macaque, Eq, Po, BWB, ICC, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB2301-25UL
DescriptionAnti-Calnexin (rabbit polyclonal)
Alternate Names
  • Calnexin
  • IP90
  • Major histocompatibility complex class I antigen-binding protein p88
  • p90
Background InformationCalnexin is a Ca++ binding protein found in ER transmembranes that binds to specific n-linked oligosaccharides found on newly synthesized proteins. Calnexin associates with the ER membrane phosphoglycoprotein, signal sequence receptor SSRμ, and has been identified as the ER protein associated with major histocompatibility complex (MHC) class I heavy chain and with the T-cell receptor and membrane immunoglobulin before assembly of these multimeric complexes. Hence, calnexin may represent a molecular chaperone in the ER membrane that is selective for incompletely folded oligomeric membrane proteins newly translocated to the ER.
References
Product Information
FormatAffinity Purified
Control
  • Human brain tissue lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Calnexin Antibody is an antibody against Calnexin for use in WB, IC, IH(P).
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Calnexin in NIH/3T3 cells.

Immunohistochemistry Analysis: A 1:1,000-2,000 dilution from a representative lot detected Calnexin in human brain and human prostate tissues.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human Calnexin.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis antibody recognizes Calnexin at the C-terminus.
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Chimpanzee
  • Rhesus Macaque
  • Horse
  • Pig
  • Bovine
Species Reactivity NoteDemonstrated to react with Human, Mouse, and Rat. Predicted to react with Chimpanzee, Rhesus Macaque, Equine, Porcine, and Bovine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008].
Gene Symbol
  • CANX
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.

SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

SEQUENCE SIMILARITIES: Belongs to the calreticulin family.
Molecular Weight~70 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human brain tissue lysate.

Western Blot Analysis: A 1:10,000 dilution of this antibody detected Calnexin on 10 µg of human brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB2301-25UL 04054839337758