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MABN1524 Anti-CHCHD10 Antibody, clone 11F11.2

Anti-CHCHD10 antibody, clone 11F11.2, Cat. No. MABN1524, is a highly specific mouse monoclonal antibody, that targets CHCHD10 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-CHCHD10 Antibody, clone 11F11.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: CHCHD10, C22orf16 UniProt Number: Q8WYQ3
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MABN1524
200 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1524
DescriptionAnti-CHCHD10 Antibody, clone 11F11.2
Alternate Names
  • Coiled-coil-helix-coiled-coil-helix domain-containing protein 10
  • mitochondrial
  • Protein N27C7-4
Background InformationCoiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial (UniProt: Q8WYQ3; also known as Protein N27C7-4) is encoded by the CHCHD10 (also known as C22orf16) gene (Gene ID: 400916) in human. CHCHD10 belongs to a family of mitochondrial proteins located in the intermembrane space that is enriched at cristae junctions. CHCHD10 displays a ubiquitous distribution pattern, but its expression is highest in heart and liver and lowest in spleen. It is involved in the maintenance of mitochondrial organization, mitochondrial cristae structure, and mitochondrial DNA stability. CHCD10 has a non-structured N-terminal region, followed by a highly hydrophobic helix and a C-terminal CHCH domain characterized by a Cx(9)C motif and 2 additional cysteines. The four cysteine residues (102, 112, 122 and 132) of the CHCH domain are involved in two disulphide bonds. Overexpression of a CHCHD10 mutant allele in HeLa cells is reported to lead to fragmentation of the mitochondrial network and ultrastructural major abnormalities including loss, disorganization and dilatation of cristae. Mutations in CHCHD10 gene are reported to cause frontotemporal dementia and/or amyotrophic lateral sclerosis 2 (FTDALS2). (Ref.: Bannwarth, S., et al. (2014). Brain. 2014 137(Pt 8):2329-2345).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CHCHD10 antibody, clone 11F11.2, Cat. No. MABN1524, is a highly specific mouse monoclonal antibody, that targets CHCHD10 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected CHCHD10 in 10 µg of human liver tissue lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CHCHD10 in human small intestine, human tonsil, human liver, and human prostate cancer tissue.
Biological Information
ImmunogenGST-tagged recombinat fragment corresponding to 79 amino acids from human CHCHD10.
Clone11F11.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 11F11.2 detects CHCHD10 in human brain and liver.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CHCHD10
  • C22orf16
Purification MethodProtein G purified
UniProt Number
Molecular Weight~15 kDa observed; 14.15 kDa calulated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CHCHD10 in 10 µg of human brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN1524 04054839153167