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04-973 Anti-CD39 Antibody, clone 5F2

This Anti-CD39 Antibody, clone 5F2 is validated for use in FC, WB for the detection of CD39.

Anti-CD39 Antibody, clone 5F2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Cytokines & Cytokine Receptors Gene Symbol: CD39, Ecto-apyrase, Ectoapyrase UniProt Number: P55772
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04-973
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFC, WBMPurifiedMonoclonal Antibody
Description
Catalogue Number04-973
DescriptionAnti-CD39 Antibody, clone 5F2
Alternate Names
  • ectonucleoside triphosphate diphosphohydrolase 1
  • ATPDase
  • ecto-apyrase
  • NTPDase-1
  • Ecto-ATP diphosphohydrolase
  • Lymphoid cell activation antigen
  • NTPDase 1
  • Ecto-apyrase
  • CD39 antigen
Background InformationEctonucleoside triphosphate diphosphohydrolase 1 (NTDase 1 or CD39) belongs to the membrane-bound ecto-nucleoside triphosphates diphosphohydrolase family (E-NTPDases) that are characterized for their ability to hydrolyze nucleoside di- and triphosphates, such as ATP, in the presence of cations, i.e. CA2+ and Mg2+. CD39 is an important membrane protein containing two transmembrane domains and a large NTPDase active, extracellular region containing apyrase conserved regions. It is heavily glycosolated, having six known N-glycosolation sites, and functions on the plasma membrane’s outer surface. CD39 initiates the metabolism, of ATP and ADP to adenosine through a 2-step exzymatic reaction, providing extracellular adenosine for the purpose of regulating various inflammatory endpoints. It is a putative marker for B-cell activation and its expression has also been observed on NK-cells, dendritic cells, T-cells, and vascular endothelial cells. CD39 is a known inhibitor of ADP-induced platelet aggregation and may also play a role in angiogenesis.
References
Product Information
FormatPurified
Control
  • BALB/c splenocytes
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-CD39 Antibody, clone 5F2 is validated for use in FC, WB for the detection of CD39.
Key Applications
  • Flow Cytometry
  • Western Blotting
Application NotesWestern Blot Analysis: A previous lot was used by an independent laboratory in WB.
Biological Information
ImmunogenRecombinant protein corresponding to mouse CD39.
EpitopeUnknown
Clone5F2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes CD39.
IsotypeIgG1κ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD39
  • Ecto-apyrase
  • Ectoapyrase
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well.

CATALYTIC ACTIVITY: ATP + 2 H2O = AMP + 2 phosphate.

COFACTOR: Calcium (By similarity).

MAGNESIUM: (By similarity).

SUBUNIT STRUCTURE: Homodimer; disulfide-linked (By similarity).

SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).

SEQUENCE SIMILARITIES: Belongs to the GDA1/CD39 NTPase family.
Molecular Weight~ 100 kDa.
It has been published that CD39 shows molecular weights between ~70-100 kDa (Wang, T.F. et al., 1996).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in BALB/c splenocytes.

Flow Cytometry Analysis: 0.1 µg/mL of this antibody detected CD39 in BALB/c splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
04-973 04053252478628