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05-831-I Anti-Amyloid Beta (ABeta) x-42 Antibody, clone 12F4

Anti-Amyloid Beta (ABeta) x-42, clone 12F4 is an antibody targeting the Amyloid Beta (ABeta) x-42 protein, validated for use in IHC & ELISA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: APP, A4, AD1 UniProt Number: P05067
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05-831-I
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIHC, ELISAMPurifiedMonoclonal Antibody
Description
Catalogue Number05-831-I
Replaces05-831
DescriptionAnti-Amyloid Beta (ABeta) x-42 Antibody, clone 12F4
Alternate Names
  • Amyloid beta A4 protein
  • ABPP
  • APPI
  • APP
  • Alzheimer disease amyloid protein
  • Cerebral vascular amyloid peptide
  • CVAP
  • PreA4
  • Protease nexin-II
  • PN-II
Background InformationOne of the most important and initial steps which causes loss of memory and cognition in Alzheimer’s Disease (AD) involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 amino acid peptides (Amyloid Beta) 1-40, 1-42 and 1-43). Polymerization of beta-amyloid and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition.
References
Product Information
FormatPurified
Control
  • Human Alzheimer's brain tissue.
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Amyloid Beta (ABeta) x-42, clone 12F4 is an antibody targeting the Amyloid Beta (ABeta) x-42 protein, validated for use in IHC & ELISA.
Key Applications
  • Immunohistochemistry
  • Enzyme Immunoassay (ELISA)
Application NotesImmunohistohemistry Analysis: A 1:500 dilution from a representative lot detected Amyloid Beta (ABeta) x-42 in human pons/midbrain tissue.

ELISA Analysis: A representative lot detected Amyloid Beta (ABeta) x-42, but demonstrates a loss of signal against Amyloid Beta (ABeta) x-42.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Amyloid Beta (ABeta) x-42, but demonstrated a loss of signal against x-40 and not x-43 in WB (Prof. J. Buxbaum, Mt. Sinai School of Medicine).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Amyloid Beta (ABeta) x-42.
Clone12F4
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody detects Amyloid Beta (ABeta) x-42, but does not cross react with Amyloid Beta (ABeta) x-40 or Amyloid Beta (Abeta) x-43.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • APP
  • A4
  • AD1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight4 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human Alzheimer's disease brain tissue.

Immunohistochemistry Analysis: A 1:2,000 dilution of this antibody detected Amyloid Beta (Abeta) x-42 in human Alzheimer's disease brain tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-831-I 04053252854774

Documentation

Anti-Amyloid Beta (ABeta) x-42 Antibody, clone 12F4 SDS

Title

Safety Data Sheet (SDS) 

Anti-Amyloid Beta (ABeta) x-42 Antibody, clone 12F4 Certificates of Analysis

TitleLot Number
Anti-Amyloid Beta (ABeta) x-42, clone 12F4 - Q2129762 Q2129762
Anti-Amyloid Beta (ABeta) x-42, -2520499 2520499
Anti-Amyloid Beta (ABeta) x-42, -2547766 2547766
Anti-Amyloid Beta (ABeta) x-42, -2548731 2548731
Anti-Amyloid Beta (ABeta) x-42, -2567139 2567139
Anti-Amyloid Beta (ABeta) x-42, -2583433 2583433
Anti-Amyloid Beta (ABeta) x-42, -2616196 2616196
Anti-Amyloid Beta (ABeta) x-42, -2651999 2651999
Anti-Amyloid Beta (ABeta) x-42, -2768480 2768480
Anti-Amyloid Beta (ABeta) x-42, clone 12F4 - 3071653 3071653
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Newsletters / Publications

Title
Research Focus - Volume 2, 2013