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ABC974-I-100UG Anti-ATG8

Anti-ATG8 Antibody, Cat. No. ABC974-I, is a rabbit polyclonal antibody that detects ATG8a and ATG8b and has been tested for use in Western Blotting.

ABC974-I-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABC974-I-100UG
DescriptionAnti-ATG8
Alternate Names
  • Autophagy-related 8a
  • isoform A and Autophagy-related 8b
  • isoform B
Background InformationAutophagy-related 8a, isoform A and Autophagy-related 8b, isoform B (UniProt: Q9W2S2 and Q9VEG5, respectively) are encoded by the ATG8a and ATG8b genes (Gene ID: 32001 and 42132, respectively) in Drosophila melanogaster. Atg8 is a highly conserved and ubiquitously expressed protein that is upregulated under various stress conditions. Atg8 genes of multicellular animals is divided into three subfamilies LC3, GABARAP, GATE16 (depending on the animal). LC3 is the mammalian ortholog of Drosophila Atg8. Atg8 It shares a strong structural similarity to ubiquitin, despite the lack of similarity in amino acid sequence. The ubiquitin core of the Atg8 consists of a four-stranded central beta sheet, where the two central strands are parallel to each other and the two outer strands are antiparallel to the central strands. Between these strands are two alpha helices with alpha3 between beta2 and beta3 and alpha4 between beta3 and beta4. In resting cells, the majority of Atg8 is in found in an unlipidated state (Atg8-I) and during autophagy it is conjugated with a lipid moiety (Atg8-II). The lipidated form is the one that is recruited to autophagosomal membranes. The level of Atg8-II is an indicator of autophagosome activation. (Ref.: Shpilka, T., et al. (2011). Genome Biology. 12(7); 226).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-ATG8 Antibody, Cat. No. ABC974-I, is a rabbit polyclonal antibody that detects ATG8a and ATG8b and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptides corresponding to 15 amino acids from the N-terminal region of Drosophila melanogaster ATG8a and ATG8b.
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects ATG8a and ATG8b in Drosophila melanogaster. It targets an epitope within 15 amino acids from the N-terminal region.
IsotypeIgG
Species Reactivity
  • Drosophila
Species Reactivity NoteDrosophila.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ATG8a
  • ATG8b
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~ 14 kDa observed; 14.39 and 14.46 kDa calculated for ATG8a and ATG8b, respectively. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in DSL2 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected ATG8a and ATG8b in DSL2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC974-I-100UG 04061841099017