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MABS1685 Anti-ARHGEF28 Antibody, clone 14C10.1

Detect ARHGEF28 using this mouse monoclonal Anti-ARHGEF28, clone 14C10.1, Cat. No. MABS1685, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-ARHGEF28 Antibody, clone 14C10.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Arhgef28, Rgnef UniProt Number: P0C6P5
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MABS1685
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1685
DescriptionAnti-ARHGEF28 Antibody, clone 14C10.1
Alternate Names
  • Rho guanine nucleotide exchange factor 28
  • 190 kDa guanine nucleotide exchange factor
  • p190-RhoGEF
  • p190RhoGEF
  • Rho guanine nucleotide exchange factor
Background InformationRho guanine nucleotide exchange factor 28 (UniProt P0C6P5; also known as 190 kDa guanine nucleotide exchange factor, p190-RhoGEF, p190RhoGEF, Rho guanine nucleotide exchange factor) is encoded by the Arhgef28 (also known as Rgnef) gene (Gene ID 361882) in rat species. p190RhoGEF is an ubiquitously expressed protein that belongs to the same subclass of Dbl family GEFs as Lbc, Lfc, GEFH-1, and p115RhoGEF. It contains several potential regulatory motifs, including an N-terminal leucine-rich region, a cysteine-rich zinc-finger domain and a C-terminal region that may form an alpha-helical coiled-coil. p190RhoGEF binds to RhoA, but not with Rac and Cdc42. When overexpressed in neuronal cells, it induces cell rounding and inhibits neurite outgrowth. Endogenous p190RhoGEF is reported to localize to distinct RhoA-containing regions at the plasma membrane, to the cytosol and along microtubules. In vitro and in vivo binding experiments show that p190RhoGEF directly interacts with microtubules via its C-terminal region adjacent to the catalytic Dbl homology/pleckstrin homology domain. Ref.: Van Horck FP et al. (2001). J. Biol. Chem. 276, 4948-4956.
References
Product Information
FormatPurified
PresentationPurified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect ARHGEF28 using this mouse monoclonal Anti-ARHGEF28, clone 14C10.1, Cat. No. MABS1685, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ARHGEF28 in rat heart and kidney tissue sections.
Biological Information
ImmunogenGST-tagged recombinant rat ARHGEF28 fragment encompassing the N-terminal portion of microtubles-interacting domain, excluding sequence C-terminal to the RNA-binding region.
Clone14C10.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 14C10.1 targets an epitope within the N-terminal portion of ARHGEF28 microtubles-interacting domain.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Rat
Species Reactivity NoteMouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Arhgef28
  • Rgnef
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~190 kDa observed. 190.8 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ARHGEF28 in 10 µg of PC12 rat pheochromocytoma cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1685 04054839092169