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AB4235 Anti-AMACR Antibody

Detect AMACR using this Anti-AMACR Antibody validated for use in WB, IH(P).

Anti-AMACR Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Tumor Markers Gene Symbol: AMACR, RACE, EC 5.1.99.4 UniProt Number: Q9UHK6
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AB4235
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB4235
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-AMACR Antibody
Alternate Names
  • a-methylacyl-CoA racemase
  • P504S
Background InformationProstate-specific antigen (PSA) screening for prostate cancer is now widespread in the United States among men of all ages. However PSA has limited specificity because benign disease, including prostatic enlargement and inflammation, can increase PSA levels. Thus, a more specific prostate cancer markers is needed. One such potential marker is AMACR, an enzyme that is involved in peroxisomal beta-oxidation of dietary branched-chain fatty acids. Recent studies have shown that, compared with expression in normal or benign prostate epithelium, AMACR is consistently overexpressed in prostate cancer epithelium, making it a specific marker for cancer cells within the prostate gland. Furthermore, overexpression of AMACR may increase the risk of prostate cancer because its expression is increased in premalignant lesions (prostatic intraepithelial neoplasia).
References
Product Information
FormatAffinity Purified
PresentationPeptide affinity purified antibody. Liquid in PBS, with 0.02% sodium azide as a preservative.
Quality LevelMQ300
Applications
ApplicationDetect AMACR using this Anti-AMACR Antibody validated for use in WB, IH(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern blot: 1:500

Immunohistochemistry: 1:100 in an ABC method. An incubation period of 30 minutes at room temperature is suggested. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenSynthetic human AMACR peptide.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes human AMACR (alpha-methylacyl-CoA racemase).
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryAlpha-methylacyl-CoA racemase is responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers, which are the only stereoisomers that can be degraded via peroxisomal beta-oxidation.[supplied by OMIM]
Gene Symbol
  • AMACR
  • RACE
  • EC 5.1.99.4
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9UHK6 # Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.
SIZE: 382 amino acids; 42360 Da
SUBCELLULAR LOCATION: Peroxisome. Mitochondrion.
DISEASE: SwissProt: Q9UHK6 # Defects in AMACR are the cause of AMACR deficiency [MIM:604489]. It results in elevated concentrations of pristanic acid. It is associated with a form of adult onset sensory motor neuropathy. & Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4) [MIM:214950]; also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.
SIMILARITY: SwissProt: Q9UHK6 ## Belongs to the caiB/baiF CoA-transferase family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8ºC in undiluted aliquots for 3 months or at -20°C for up to one year from date of receipt. Avoid repeated freeze-thaw cycles.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB4235 04053252508134

Documentation

Anti-AMACR Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-AMACR Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-AMACR (P504S) POLYCLONAL ANTIBODY - 2368696 2368696
RABBIT ANTI-AMACR (P504S) - 3286444 3286444
RABBIT ANTI-AMACR (P504S) POLYCLONAL ANTIBODY 3063422
RABBIT ANTI-AMACR (P504S) POLYCLONAL ANTIBODY - 2361783 2361783
RABBIT ANTI-AMACR (P504S) POLYCLONAL ANTIBODY - 2365780 2365780

References

Reference overviewPub Med ID
P504S immunohistochemical detection in 405 prostatic specimens including 376 18-gauge needle biopsies.
Beach, R, et al.
Am. J. Surg. Pathol., 26: 1588-96 (2002)  2002

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