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MABE317 Sigma-AldrichAnti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01

Detect 5-Hydroxymethylcytosine (5hmC) using this Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01 validated for use in Dot Blot, ICC, Immunoprecipitation.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology

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Overview

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
A	DB, ICC, IP	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABE317
Description	Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01
Alternate Names	5hmeC 5' hydroxymethyl cytosine 5'OH methyl cytosine 5'OHmeC
Background Information	5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.

Product Information
Format	Purified
Control	Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Detect 5-Hydroxymethylcytosine (5hmC) using this Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01 validated for use in Dot Blot, ICC, Immunoprecipitation.
Key Applications	Dot Blot Immunocytochemistry Immunoprecipitation
Application Notes	Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Analysis: A representative lot was used by an independent laboratory to immunoprecipitate 5-Hydroxymethylcytosine DNA usingh chomatin from 293T cells overexpressing mTET1-CD. (Dr. D. Chen, Shanghai Institute of Biological Sciences) Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to stain 5-Hydroxymethylcytosine DNA in cortical neurons from atg5-disrupted mouse. (Dr. D. Chen, Shanghai Institute of Biological Sciences)

Biological Information
Immunogen	BSA-conjugated recombinant Hydroxymethylcytosine DNA.
Clone	HMC-MA01
Host	Mouse
Specificity	This antibody detects 5-Hydroxymethylcytosine, but not 5-Methylcytosine or unmethylated cytosine.
Isotype	IgG1κ
Species Reactivity	All
Species Reactivity Note	Expected to detect 5-Hydroxymethylcytosine in all species
Antibody Type	Monoclonal Antibody
Purification Method	Protein G Purified

Product Usage Statements
Quality Assurance	Evaluated by Dot Blot in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-Hydroxymethylcytosine DNA. Dot Blot Analysis: A 1:5000 dilution of this antibody detected only 5-hydroxymethylcytosine in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Global Trade Item Number
Catalogue Number	GTIN
MABE317	04053252551857

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