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ST1053 Anti-20S Proteasome Core Subunits Rabbit pAb

This Anti-20S Proteasome Core Subunits Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of 20S Proteasome Core Subunits.

Anti-20S Proteasome Core Subunits Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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ST1053
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, YeastRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
ST1053-100ULCN Plastic ampoule 100 ul
Description
OverviewRecognizes the ~25-30 kDa 20S proteasome core subunits (α5, α7, β1, β5i, and β7) in human erythrocytes, HeLa cells and 3T3 cells.
Catalogue NumberST1053
Brand Family Calbiochem®
Application Data
Detection of human 20S proteasome core subunits by immunoblotting. Sample: Whole cell lysate from human erythrocytes. Primary antibody: Anti-20S Proteasome Core Subunits Rabbit pAb (Cat. No. ST1053) (1:1000). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationSerum diluted in PBS.
Positive controlHeLa cells, RSV 3T3 mouse fibroblasts, or yeast whole cell lysates
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Gong, X., et al. 2007. Cancer Res. 67, 5380.
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (1:1000-1:10,000, see application references)
Immunoprecipitation (see comments)
Paraffin Sections (1:100)
Application CommentsThis antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenhuman erythrocyte proteasomes
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Yeast
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Irritant
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1053-100ULCN 04055977224382

Documentation

Anti-20S Proteasome Core Subunits Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-20S Proteasome Core Subunits Rabbit pAb Certificates of Analysis

TitleLot Number
ST1053
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-July-2007
ApplicationImmunoblotting (1:1000-1:10,000, see application references)
Immunoprecipitation (see comments)
Paraffin Sections (1:100)
Application Data
Detection of human 20S proteasome core subunits by immunoblotting. Sample: Whole cell lysate from human erythrocytes. Primary antibody: Anti-20S Proteasome Core Subunits Rabbit pAb (Cat. No. ST1053) (1:1000). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody supplied as diluted serum. Recognizes the ~27-30 kDa 20S proteasome core subunits (α5, α7, β1, β5i, and β7).
BackgroundProteasomes are large multi-subunit complexes, that are localized in the nucleus and cytosol, and selectively degrade intracellular proteins. They play a major role in the degradation of many proteins involved in cellular processes such as cell cycling, apoptosis, and proliferation. The human proteasome contains two copies of each of seven different α-type and β-type subunits assembled to form a stack of four seven-membered rings. The α-subunits comprise the outer rings while the β-subunits comprise the inner rings. The β-subunits are believed to be responsible for the proteolytic activity of the 20S proteasome.
HostRabbit
Immunogen speciesHuman
Immunogenhuman erythrocyte proteasomes
IsotypeIgG
Specieshuman, mouse, yeast
Positive controlHeLa cells, RSV 3T3 mouse fibroblasts, or yeast whole cell lysates
FormLiquid
FormulationSerum diluted in PBS.
Preservative≤0.1% sodium azide
CommentsThis antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Irritant
Application referencesImmunoblotting Gong, X., et al. 2007. Cancer Res. 67, 5380.