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AB9748-I Anti-14-3-3 Antibody (pan)

Anti-14-3-3 pan, Cat. No. AB9748-I, is a highly specific rabbit polyclonal antibody, that targets 14-3-3 protein and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting and .

Anti-14-3-3 Antibody (pan) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: YWHAB, YWHAE, YWHAG, YWHAZ, YWHA1, YWHAH, YWHAQ UniProt Number: P31946, P63104, P62258, Q04917, P61981, P27348
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AB9748-I
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIP, WB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB9748-I
ReplacesAB9748
DescriptionAnti-14-3-3 Antibody (pan)
Alternate Names
  • 14-3-3 protein beta/alpha
  • KCIP-1
  • Protein 1054
  • Protein kinase C inhibitor protein 1
  • 14-3-3 protein epsilon
  • 14-3-3E
  • 14-3-3 protein eta
  • Protein AS1
  • 14-3-3 protein gamma
  • 14-3-3 protein theta
  • 14-3-3 protein T-cell
  • 14-3-3 protein tau
  • Protein HS1
  • 14-3-3 protein zeta/delta
Background Information14-3-3 protein beta/alpha (KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1), epsilon (14-3-3E), eta (Protein AS1), gamma (KCIP-1, Protein kinase C inhibitor protein 1), theta (14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1), zeta/delta (KCIP-1, Protein kinase C inhibitor protein 1) are encoded by the YWHAB, YWHAE, YWHAH, YWHAG, YWHAQ, YWHAZ gene (Gene ID 7529, 7531, 7533, 7532, 10971, 7534, respectively) in human. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer's. The 14-3-3 pan antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer's and Parkinson's disease.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-14-3-3 pan, Cat. No. AB9748-I, is a highly specific rabbit polyclonal antibody, that targets 14-3-3 protein and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting and .
Key Applications
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:5,000 dilution from a representative lot detected 14-3-3 immunoreactivity in human prostate cancer, cerebellum, and tonsil tissue sections.

Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated 14-3-3 from 500 µg of A431 cell lysate.
Biological Information
ImmunogenA mixture of KLH-conjugated linear peptides corresponding the the N-terminal end sequences of human 14-3-3 alpha/beta (YWHAB), gamma (YWHAG), zeta/delta (YWHAZ), epsilon (YWHAE), eta (YWHAH or YWHA1), and theta (YWHAQ).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis antibody targets the N-terminal end sequences of 14-3-3 alpha/beta (YWHAB), gamma (YWHAG), zeta/delta (YWHAZ), epsilon (YWHAE), eta (YWHAH or YWHA1), and theta (YWHAQ).
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Chicken, Bovine, Fish, Porcine, Rabbit, Mouse, Rat, Sheep, Xenopus, Zebra Fish based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • YWHAB
  • YWHAE
  • YWHAG
  • YWHAZ
  • YWHA1
  • YWHAH
  • YWHAQ
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~28 kDa observed. 28.08/27.85 kDa (human isoform Long/Short; YWHAB), 27.75 kDa (human YWHAZ), 29.17 kDa (human YWHAE), 28.22 kDa (human YWHAH or YWHA1), 28.30 kDa (human YWHAG), 27.76 kDa (human YWHAQ) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected 14-3-3 in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB9748-I 04054839088933