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565781 β-Secretase Substrate VI, Fluorogenic - Calbiochem

A highly selective, fluorescence resonance energy transfer (FRET) peptide substrate for β-secretase (BACE); (pH ~4.0, kcat = 0.02 sec⁻¹; Km = 9 µM).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: H-K(DABSYL)-SEVNLDAEFRQ(LY)

565781
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
565781-500UGCN Plastic ampoule 500 μg
Description
OverviewA highly selective, fluorescence resonance energy transfer (FRET) peptide substrate for β-secretase (BACE); (pH ~4.0, kcat = 0.02 sec-1; Km = 9 µM). Derived from the Swedish mutant APP (amyloid precursor protein) β-cleavage site (-NL↓DA-). Useful for high throughput screening of β-secretase inhibitors.
Catalogue Number565781
Brand Family Calbiochem®
SynonymsH-K(DABSYL)-SEVNLDAEFRQ(LY)
References
ReferencesGrüninger-Leitch, F., et al. 2002. J. Biol. Chem. 277, 4687.
Product Information
ATP CompetitiveN
FormLyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₈₉H₁₂₂N₂₄O₃₁S₃
Chemical formulaC₈₉H₁₂₂N₂₄O₃₁S₃
Hygroscopic Hygroscopic
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetFluorescence resonance energy transfer (FRET) peptide substrate for β-secretase (BACE)
Primary Target IC<sub>50</sub>kcat = 0.02 sec⁻¹ and Km = 9 µM at pH ~4.0 as fluorescence resonance energy transfer (FRET) peptide substrate for β-secretase (BACE)
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceH-Lys(DABSYL)-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Gin-(LY)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
565781-500UGCN 04055977267297

Documentation

β-Secretase Substrate VI, Fluorogenic - Calbiochem Certificates of Analysis

TitleLot Number
565781

References

Reference overview
Grüninger-Leitch, F., et al. 2002. J. Biol. Chem. 277, 4687.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-September-2008 RFH
SynonymsH-K(DABSYL)-SEVNLDAEFRQ(LY)
DescriptionA highly selective, fluorescence resonance energy transfer (FRET) peptide substrate for b-secretase (BACE) (pH ~4.0, kcat = 0.02 sec-1; Km = 9 µM). Derived from the Swedish mutant APP (amyloid precursor protein) β-cleavage site (-NL ↓ DA-). Useful for high throughput screening of β-secretase inhibitors. Excitation max.: ~430 nm; emission max.: ~520 nm.
FormLyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₈₉H₁₂₂N₂₄O₃₁S₃
Peptide SequenceH-Lys(DABSYL)-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Gin-(LY)
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml)
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Toxicity Standard Handling
ReferencesGrüninger-Leitch, F., et al. 2002. J. Biol. Chem. 277, 4687.