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203390 (±)-Blebbistatin - CAS 674289-55-5 - Calbiochem

(±)-Blebbistatin, CAS 674289-55-5, is a cell-permeable, selective, and reversible inhibitor of nonmuscle myosin II. Blocks cell blebbing and disrupts cell migration & cytoKinesis in vertebrate cells.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: ATPase
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203390
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
674289-55-5C₁₈H₁₆N₂O₂

Products

Catalogue NumberPackaging Qty/Pack
203390-5MGCN Plastic ampoule 5 mg
Description
OverviewA cell-permeable compound that acts as a selective, potent, and reversible inhibitor of nonmuscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤ 100 µM) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly. A 50 mM (5 mg/342 µl) solution of (±)-Blebbistatin (Cat. No. 203389) in 90% DMSO is also available.
Catalogue Number203390
Brand Family Calbiochem®
References
ReferencesShu, S., et al. 2005. Proc. Natl. Acad. Sci. USA 102, 1472.
Kovacs, M., et al. 2004. J. Biol. Chem. 279, 35557.
Straight, A.F., et al. 2003. Science 299, 1743.
Cheung, A., et al. 2001. Mol. Biol. Cell Suppl. 12, 271a.
Product Information
CAS number674289-55-5
ATP CompetitiveN
FormYellow solid
Hill FormulaC₁₈H₁₆N₂O₂
Chemical formulaC₁₈H₁₆N₂O₂
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetATPase
Primary Target IC<sub>50</sub><100 µM inhibiting the ATPase and gliding motility of human platelets
Purity≥97% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution in 90% DMSO, aliquot and freeze (-20°C). Stock solutions in 90% DMSO are stable for up to 1 month at -20°C. Stock solutions in 100% DMSO are unstable; reconstitute just prior to use.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
203390-5MGCN 04055977221268

Documentation

(±)-Blebbistatin - CAS 674289-55-5 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

(±)-Blebbistatin - CAS 674289-55-5 - Calbiochem Certificates of Analysis

TitleLot Number
203390

References

Reference overview
Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA 102, 1472.
Kovacs, M., et al. 2004. J. Biol. Chem. 279, 35557.
Straight, A.F., et al. 2003. Science 299, 1743.
Cheung, A., et al. 2001. Mol. Biol. Cell Suppl. 12, 271a.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision31-March-2011 RFH
DescriptionA cell-permeable compound that acts as a selective, potent, and reversible inhibitor of non-muscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤100 µM) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly.
FormYellow solid
Intert gas (Yes/No) Packaged under inert gas
CAS number674289-55-5
Chemical formulaC₁₈H₁₆N₂O₂
Structure formulaStructure formula
Purity≥97% by HPLC
Solubility100% DMSO (100 mg/ml), 90% DMSO (75 mg/ml) or Methanol (1.5 mg/ml)
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution in 90% DMSO, aliquot and freeze (-20°C). Stock solutions in 90% DMSO are stable for up to 1 month at -20°C. Stock solutions in 100% DMSO are unstable; reconstitute just prior to use.
ReferencesShu, S., et al. 2005. Proc. Natl. Acad. Sci. USA 102, 1472.
Kovacs, M., et al. 2004. J. Biol. Chem. 279, 35557.
Straight, A.F., et al. 2003. Science 299, 1743.
Cheung, A., et al. 2001. Mol. Biol. Cell Suppl. 12, 271a.