elispot
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ELISpot Optimization | Life Science Research | Merck
The use of highly specific ELISpot-validated Ab pairs is key to assay success. -
Detection | Life Science Research | Merck
ELISpot assays may be performed either with antibodies directly conjugated to the detection motif (enzyme or fluorochrome) or as a two-step process involving a biotin/streptavidin-conjugated Ab pair. -
Cells | Life Science Research | Merck
The key to consistency in an ELISpot assay is to use stable cell cultures and develop good cell handling techniques. -
ELISpot Learning Center | Life Science Research | Merck
ELISpot assays offer multi-dimensional, quantitative assessment of effector function(s) at the single cell level with superior sensitivity and resolution. -
Plates | Life Science Research | Merck
Learn about the key factors in developing a successful ELISpot assay: from plate chemistry, to layout of controls, to plate handling practices. -
ELISpot Advantages | Life Science Research | Merck
While many assay formats exist for studying ex vivo T cell effector function, ELISpot assays offer clear advantages. -
Spot Counting and Analysis - What Is a Real Spot? | Life Science Research | Merck
True spot has a densely colored center which fades toward the edges the size and/or color intensity of the spots is determined by the amount of cytokine released. -
The Standard ELISpot Assay Steps | Life Science Research | Merck
Learn about the standard ELISpot Assay process and how ELISpots are currently performed on polyvinylidene fluoride (PVDF) membrane plates. -
Troubleshooting Your ELISpot Assay | Life Science Research | Merck
Answers to help troubleshoot your ELISpot Assay