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The following MAPmates™ should not be plexed together: -MAPmates™ that require a different assay buffer -Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9) -PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701) -More than 1 phospho-MAPmate™ for a single target (Akt, STAT3) -GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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70771
pET Expression System 25b
pET vectors plus host strains with induction control
More>>pET vectors plus host strains with induction control Less<<
pET Expression System 25b: Malzeme Güvenlik Bilgi Formu (MSDS) veya SDS, Analiz Sertifikası (COA) ve Kalite Uygunluk Sertifikası (COQ), dosyalar, broşürler ve diğer dokümanlar.
Diethyl Pyrocarbonate - CAS 1609-47-8 - Calbiochem
Overview
Replacement Information
Description
Overview
This product has been discontinued.
pET Expression Systems and pET pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.
Components: pET Expression Systems Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
• 10 µg pET vector DNA (for each indicated plasmid)
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BL21(DE3) Glycerol Stock
• 0.2 ml BL21(DE3)pLysS Glycerol Stock
• 0.2 ml Induction Control Glycerol Stock
Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
• 0.2 ml NovaBlue Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 2 × 0.2 ml SOC Medium
• 10 µl Test Plasmid
These components are sufficient for up to 10 transformations in each host.
Purification and Detection Reagents Purification and detection reagents are
available separately. For complete product descriptions and ordering information, refer
to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.
pET Expression System 25b
The pET-25b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal HSV•Tag® and His•Tag® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB065VM.
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Catalogue Number
70771
Brand Family
Novagen®
References
Product Information
•
10 µg
pET-25b(+) vector DNA
•
0.2 ml
Host bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
•
0.2 ml
Induction control clone, glycerol stock
Fusion tag
His•Tag
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Shipped with Blue Ice or with Dry Ice
Toxicity
Standard Handling
Storage
≤ -70°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number
GTIN
70771
0
Documentation
pET Expression System 25b Certificates of Analysis
Hao Zhou, Wei Wang and Yongzhang Luo. (2005) Contributions of disulfide bonds in a nested pattern to the structure, stability, and biological functions of endostatin. Journal of Biological Chemistry280, 11303-11312.
P. M. Deckert, et al. (2003) A33scFv-cytosine deaminase: a recombinant protein construct for antibody-directed enzyme-prodrug therapy. British Journal of Cancer88, 937-939.
Songmin Cai and John H. Exton. (2001) Determination of interaction sites of phospholipase D1 for RhoA. Biochemical Journal355, 779-785.
Kerri Cooper and Francois Baneyx. (2001) Escherichia coli FtsH (HflB) degrades a membrane-associated TolAI-II-βlactamase fusion protein under highly denaturing conditions. Protein Expression and Purification21, 323-332.
Michael W. West, et al. (1999) De novo amyloid proteins from designed combinatorial libraries. Procedings of the National Academy of Science96, 1121111216.
L.L. Murley and N.D.F. Grindley. (1998) Architecture of the γδ resolvase synaptosome: oriented heterodimers identify interactions essential for synapsis and recombination. Cell95, 553-562.
Paul Zhou, et al. (1998) Cells transfected with a non-neutralizing antibody gene are resistant to HIV infection: trageting the endoplasmic reticulum and trans-golgi network. Journal of Immunology160, 1439-1496.
