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Merck

ST1053

Anti-20S Proteasome Core Subunits Rabbit pAb

liquid, Calbiochem®

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
yeast, human, mouse
Application:
Citations:
3

Key Documents

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Product Name

Anti-20S Proteasome Core Subunits Rabbit pAb, liquid, Calbiochem®

biological source

rabbit

antibody form

diluted serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

yeast, human, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (1:1000-1:10,000,
Immunoprecipitation
Paraffin Sections (1:100))

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... PSMB2(5690)

Analysis Note

Positive Control
HeLa cells, RSV 3T3 mouse fibroblasts, or yeast whole cell lysates

Application


Immunoblotting (1:1000-1:10,000, see application references)
Immunoprecipitation (see comments)
Paraffin Sections (1:100)

Disclaimer

Toxicity: Irritant (B)

General description

Rabbit polyclonal antibody supplied as diluted serum. Recognizes the ~27-30 kDa 20S proteasome core subunits (α5, α7, β1, β5i, and β7).
Recognizes the ~25-30 kDa 20S proteasome core subunits (α5, α7, β1, β5i, and β7) in human erythrocytes, HeLa cells and 3T3 cells.
This Anti-20S Proteasome Core Subunits Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of 20S Proteasome Core Subunits.

Immunogen

Human
human erythrocyte proteasomes

Other Notes

This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Physical form

Serum diluted in PBS.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Junkang Xu et al.
The Journal of biological chemistry, 287(50), 41608-41618 (2012-10-23)
NAD(P)H:quinone oxidoreductase 1 (NQO1) is a flavoprotein that protects cells against radiation and chemical-induced oxidative stress. Disruption of NQO1 gene in mice leads to increased susceptibility to myeloproliferative disease. In this report, we demonstrate that NQO1 controls the stability of
Tao Li et al.
Frontiers in pharmacology, 9, 377-377 (2018-05-05)
Parkinson's disease (PD) is characterized by the loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNc) and the presence of Lewy bodies (LBs) in the surviving SNc neurons. LBs formation is caused by the accumulation of α-synuclein
Andrew P Koutnik et al.
Journal of cachexia, sarcopenia and muscle, 11(4), 973-996 (2020-04-03)
Cancer Anorexia Cachexia Syndrome (CACS) is a distinct atrophy disease negatively influencing multiple aspects of clinical care and patient quality of life. Although it directly causes 20% of all cancer-related deaths, there are currently no model systems that encompass the

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