454849
MAP Kinase 1, His•Tag®, Human, Recombinant, E. coli
Synonym(s):
ERK1, MAPK3, p44 MAPK
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
NACRES:
NA.54
UNSPSC Code:
12352202
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
liquid
specific activity
≥6000 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
foreign activity
Proteases, none detected
shipped in
wet ice
storage temp.
−70°C
Quality Level
Disclaimer
Toxicity: Standard Handling (A)
General description
Recombinant, human MAP Kinase 1 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of purified, recombinant MAP kinase in vitro with MEK1. Suitable for labeling MAP kinase substrates or for inhibitor screening.
Other Notes
Charest, D.L., et al. 1993. Mol. Cell Biol. 13, 4679.
One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
Physical form
In 100 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Stephanie Belanger-Jasmin et al.
Journal of neurochemistry, 103(5), 2022-2034 (2007-09-18)
Hairy/Enhancer of split (Hes) 6 is a basic helix-loop-helix protein that interacts with the transcriptional co-repressor, Groucho, and antagonizes the neural functions of the Notch pathway. More specifically, mouse Hes6 regulates cerebral corticogenesis by promoting neurogenesis and suppressing astrocyte differentiation.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service