Collagenase, Type I, Clostridium histolyticum

Name: Collagenase, Type I, <i>Clostridium histolyticum</i>
Brand: MM

Collagenase, Type I, Clostridium histolyticum, CAS 9001-12-1, is a crude preparation that contains several collagenases, a sulfhydryl protease, clostripain, and trypsin-like proteolytic activities.

Synonym(s):

Collagenase, Type I, Clostridium histolyticum

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About This Item

CAS Number:

9001-12-1

EC Number:

3.4.24.3 (BRENDA, IUBMB)

UNSPSC Code:

12352202

MDL number:

MFCD00130830

Key Documents

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Product Name

Collagenase, Type I, Clostridium histolyticum, Collagenase, Type I, Clostridium histolyticum, CAS 9001-12-1, is a crude preparation that contains several collagenases, a sulfhydryl protease, clostripain, and trypsin-like proteolytic activities.

form

lyophilized

specific activity

≥125 ABC units/mg solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
desiccated (hygroscopic)

solubility

aqueous buffer: 1 mg/mL

foreign activity

Caseinase ≤100 FFC unit/500 ABC unit

shipped in

ambient

storage temp.

2-8°C

Quality Level

100

Disclaimer

Toxicity: Harmful (C)

General description

Crude enzyme preparation that contains several collagenases, a sulfhydryl protease, clostripain and trypsin-like proteolytic activities. Especially useful for tissue dissociation studies. Most active on fat cells, adrenal and liver tissue, with an optimal pH of 7.0-8.0.

Crude preparation that contains several collagenases, a sulfhydryl protease, clostripain, and trypsin-like proteolytic activities. Especially useful for tissue dissociation studies.

Note: 1 ABC unit = 1.1 BTC unit.

Other Notes

Harris, E.D., Jr., and Krane, S.M. 1974. N. Engl. J. Med.291, 557.
Seifter, S., and Harper, E. 1970. Methods Enzymol.19, 613.

One ABC unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent per min from collagen at 37°C, pH 7.2. Note: 1 ABC unit = 1.1 BTC (bovine tendon collagen) unit.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Collagenase, Type I, Clostridium histolyticum