Millipore Sigma Vibrant Logo

QIA10 c-ErbB2/c-Neu Rapid Format ELISA Kit

c-ErbB2/c-Neu Rapid Format ELISA Kit: Malzeme Güvenlik Bilgi Formu (MSDS) veya SDS, Analiz Sertifikası (COA) ve Kalite Uygunluk Sertifikası (COQ), dosyalar, broşürler ve diğer dokümanlar.

Synonyms: c-Neu ELISA Kit, ErbB2 ELISA Kit, HER2 ELISA Kit, Erythroblastosis Virus ELISA Kit, HER-2 ELISA Kit

QIA10
  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Key Spec Table

      Species Reactivity
      H
      Description
      Overview

      This product has been discontinued.



      Detects both c-ErbB2/c-Neu p185 and the extracellular domain p105 cleavage fragment. Positive cell lines: A549, HT29, SK-BR-3, and SKOV-3, or breast, prostate, colon, or ovarian cancer.
      Catalogue NumberQIA10
      Brand Family Calbiochem®
      Synonymsc-Neu ELISA Kit, ErbB2 ELISA Kit, HER2 ELISA Kit, Erythroblastosis Virus ELISA Kit, HER-2 ELISA Kit
      Application Data

      In a limited study (30 cancer and 30 normal sera samples), the assay recognized 17% of cancer samples as positive and 95% of normal samples as negative.
      Materials Required but Not Delivered• 2-20 µl, 20-200 µl and 200-1000 µl precision pipetters with disposable tips.
      • Wash bottle or multichannel dispenser for washing.
      • 500 ml graduated cylinder.
      • Deionized or distilled H2O.
      • Spectrophotometer capable of measuring absorbance in 96-well plates using dual wavelength of 450/595 nm or 450/540nm.
      References
      ReferencesGauchez, A.S., et al. 2008. Anticancer Res. 28, 3067.
      Hung, M.-C. and Y-K Lau, 1999. Seminars in Oncology 26(Suppl 12), 51.
      Ouyang, X., et al. 1999. Lancet 353, 1591.
      Ross, J.S. and J.A. Fletcher, 1999. Am. J. Clin. Pathol. 112, S53.
      Ross, J.S. and J.A. Fletcher, 1998. Stem Cells 16, 413.
      Disis, M.L., et al. 1997. J. Clin. Oncol. 15, 3363.
      Ross, J.S., et al. 1997. Human Pathology 28, 827.
      Ross, J.S., et al. 1997. Cancer 79, 2162.
      Xia, W., et al. 1997. Clin. Cancer Res. 3, 3.
      Cirisano, F.D. and B.Y. Karlan, 1996. J. Soc. Gynecol. Invest. 3, 99.
      Menden, H., et al. 1994. Cancer Res. Clin. Oncol. 120, 378.
      Kynast, B., et al. 1993. J. Cancer Res. Clin. Oncol. 119, 249.
      Gusterson, B. and R. Gelber, 1992. J. Clin. Oncol. 10, 1049.
      Hetzel, D.J., et al. 1992. Gynecol. Oncol. 47, 179.
      Hou, L., et al. 1992. Cancer Lett. 65, 215.
      Leitzel, K., et al. 1992. J. Clin. Oncol. 10, 1436.
      Carney, W.P., et al. 1991. J. Tumor Marker Oncol. 6, p. 53.
      Clark, G.M. and W.L. McGuire, 1991. Cancer Res. 51, 944.
      Langton, B.C., et al. 1991. Cancer Res. 51, 2593.
      Paterson, M.C., et al. 1991. Cancer Res. 51, 556.
      Zabrecky, J.R., et al .1991. J. Biol. Chem. 266, 1716.
      Berchuck, A., et al. 1990. Cancer Res. 50, 4087.
      Borg, A., et. al. 1990. Cancer Res. 50, 4332.
      Inglehart, J.D., et al. 1990. Cancer Res. 50, 6701.
      Kern, J.A., et al. 1990. Cancer Res. 50, 5189.
      Mori, S., et al. 1990. Jpn. J. Cancer Res. 81, 489.
      Paik, S., et al. 1990. J. Clin. Oncol. 8, 103.
      Maguire, H.C. and M.I. Greene, 1989. Seminars in Oncology 16, 148.
      Ro, J., et al. 1989. Cancer Res. 49, 6941.
      Slamon, D.J., et al. 1989. Science 244, 707.
      Tandon, A.K., et al. 1989. J. Clin. Oncol. 7, 1120.
      Wright, C., et al. 1989. Cancer Res. 49, 2087.
      Yoshida, K., et al. 1989. Virchows Arch. B Cell Pathol. Mol. Pathol. 57, 285.
      Fontaine, J., et al. 1988. Oncology 45, 360.
      Kraus, M.H., et al., 1987. EMBO J. 6, 605.
      Slamon, D., et al. 1987. Science 235, 177.
      King, C.R., et al. 1985. Science 229, 974.
      Shin, C., et al. 1981. Nature 290, 261.
      Henry, R.J., et al. 1974. Clinical Chemistry, Harper and Row, New York.
      Product Information
      Form96 Tests
      Format96-well plate
      Kit containsPre-Coated 96-Well Plate, c-ErbB2/c-Neu Lyophilized Standard, Cell Resuspension Buffer, Antigen Extraction Agent, Sample Diluent, Biotinylated Detector Antibody, Detector Diluent, Streptavidin Peroxidase Conjugate, Conjugate Diluent, Wash Buffer, Substrate, Stop Solution, Adhesive Plate Sealers, and a user protocol.
      Positive controlc-erbB2/c-neu
      Biological Information
      Assay range0-3 ng/ml
      Assay time4 h
      Sample TypeCell lysates, biological fluids
      Species Reactivity
      • Human
      Safety Information
      R PhraseR: 23/24/25-35-36/37/38-40-41-43

      Toxic by inhalation, in contact with skin and if swallowed.
      Causes severe burns.
      Irritating to eyes, respiratory system and skin.
      Limited evidence of a carcinogenic effect.
      Risk of serious damage to eyes.
      May cause sensitization by skin contact.
      S PhraseS: 23-26-36/37/39-45

      Do not breathe fumes.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Intended useThe Calbiochem® c-erbB2/c-neu Rapid Format ELISA is a non-isotopic immunoassay for the in vitro quantitation of human c-erbB2/HER-2/neu oncoprotein in cell lysates, cell culture medium, sera and plasma.
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage -20°C
      Storage ConditionsUpon receipt, store the entire kit at -20°C until use. Do not refreeze after thawing. Lyophilized standards must remain at -20°C. All other components may be stored at 4°C. Do not expose reagents to excessive light. Allow kit reagents to warm to room temperature before use.
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsPre-Coated 96-Well Plate, c-ErbB2/c-Neu Lyophilized Standard, Cell Resuspension Buffer, Antigen Extraction Agent, Sample Diluent, Biotinylated Detector Antibody, Detector Diluent, Streptavidin Peroxidase Conjugate, Conjugate Diluent, Wash Buffer, Substrate, Stop Solution, Adhesive Plate Sealers, and a user protocol.
      Global Trade Item Number
      Catalogue Number GTIN
      QIA10 0