Millipore Sigma Vibrant Logo

SCC226 YUMM1.4 Mouse Melanoma Cell Line

The YUMM1.4 cell line is driven by Braf activation, Pten inactivation, and Cdkn2a inactivation. The cell line is positive for the common melanoma marker, MelanA. The cells are syngeneic with C57BL/6J mice and are very tumorigenic in vivo.

NewSCC226
>1x10^6 viable cells/vial  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information
      Description
      Catalogue NumberSCC226
      DescriptionYUMM1.4 Mouse Melanoma Cell Line
      Alternate Names
      • Yale University Mouse Melanoma cell line
      • Yale University Mouse Melanoma 1.4 cell line
      Background InformationAs immune-based therapies in cancer models become more popular and applicable, a further need for immune-competent models has been presented. A wide variety of genetically engineered mouse models have been created to express vital melanoma genotypes. The variety in these models have allowed the study of genetic alterations that are relevant to human melanoma biology. While it is quite useful to have mouse models with genetically defined human melanoma characteristics, it is limited by complex mouse colony maintenance.

      The YUMM1.4 cell line was produced to be more experimentally relevant. The cell line was derived by backcrossing important alleles into C57BL/6J mice. The mice were interbred to produce human-relevant genetically defined mouse models that could then be used to generate cell lines. Melanomas were induced by applying 4-hydroxytamoxifen to induce Cre-lox allele recombination. Cells were then mechanically and enzymatically dissociated from the removed tumor. The melanoma lines generated from this procedure retained important human melanoma characteristics useful for studying disease progression.

      The YUMM1.4 cell line is driven by Braf activation, Pten inactivation, and Cdkn2a inactivation.1 The cell line is positive for the common melanoma marker, MelanA. The cells are syngeneic with C57BL/6J mice and are very tumorigenic in vivo.

      Source
      Cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57BL/6 mouse into which mutations from the Braf/Pten genetically-engineered mouse model had been introduced via backcrossing.

      References
      1. Meeth, K., Wang, J. X., Micevic, G., Damsky, W., & Bosenberg, M. W. (2016). The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment cell & melanoma research, 29(5), 590-597.
      References
      Product Information
      Components
      • ≥1x106 viable YUMM1.4 Cells: (Catalog No. SCC226). Store in liquid nitrogen.
      Quality LevelMQ100
      Applications
      ApplicationThe YUMM1.4 cell line is driven by Braf activation, Pten inactivation, and Cdkn2a inactivation. The cell line is positive for the common melanoma marker, MelanA. The cells are syngeneic with C57BL/6J mice and are very tumorigenic in vivo.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
      Biological Information
      HostMouse
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
      • YUMM1.4 cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsYUMM1.4 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size>1x10^6 viable cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC226 04065272100830