539493
PP1, α-Isoform, Rabbit Muscle, Recombinant, E. coli
Synonym(s):
Protein Phosphatase 1, α-Isoform, Phosphorylase Phosphatase
form
liquid
specific activity
≥500 units/mL
13,500 U/mg
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
foreign activity
Protease, none detected
shipped in
wet ice
storage temp.
−70°C
Disclaimer
Toxicity: Standard Handling (A)
General description
Recombinant, rabbit, Mn2+-dependent catalytic subunit of the α-isoform of protein phosphatase 1 expressed in E. coli. Hydrolyzes the phosphate groups on serine and threonine residues, and has also been known to hydrolyze phosphates on tyrosine residues. Assists in tobacco mosaic virus-mediated programmed cell death.
Other Notes
Dunigan, D.D., and Madlener, J.C. 1995. Virology 207, 460.
Zhang, Z., et al. 1993. Mol. Cell. Biochem. 127, 113.
Zhang, A.J., et al. 1992. J. Biol. Chem. 267, 1484.
Zhang, Z., et al. 1993. Mol. Cell. Biochem. 127, 113.
Zhang, A.J., et al. 1992. J. Biol. Chem. 267, 1484.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of pNPP per minute at 30°C, pH 7.0.
Physical form
In 200 mM NaCl, 50 mM HEPES, 2.5 mM DTT, 1 mM MnCl₂, 0.1 mM EGTA, 50% glycerol, 0.025% TWEEN®-20 Detergent, pH 7.0.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Storage Class
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
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Yi-Min Chen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 844(1), 134-141 (2006-08-08)
Artemia assays and protein phosphatase assays are commonly used for the screening of microcystins (MCs) in algal samples instead of the standard mouse toxicity assay. However, it has been shown that their results are often biased because of the matrix
Thuy-Ai Nguyen et al.
DNA repair, 9(7), 813-823 (2010-05-11)
Nucleotide excision repair (NER) is the only mechanism in humans to repair UV-induced DNA lesions such as pyrimidine (6-4) pyrimidone photoproducts and cyclobutane pyrimidine dimers (CPDs). In response to UV damage, the ataxia telangiectasia mutated and Rad3-related (ATR) kinase phosphorylates
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