AAMT002 Sigma-AldrichMitochondrial Complex II Activity Assay Kit

This product has been discontinued.

Product Description

Complex II, also known as succinate-coenzyme Q reductase (SDH, EC 1.3.5.1), is one of the five complexes involved in oxidative phosphorylation in the inner mitochondrial membrane and also a member of the tricarboxylic acid cycle (TCA). It catalyzes electron transfer from succinate to the electron carrier, ubiquinone, but unlike the other four complexes it is not a proton pump. The product ubiquinol is utilized by complex III in the respiratory chain and the product fumarate is necessary to maintain the TCA cycle.

15 ml 	20X Buffer, (72581-15ML)
25 ml	Complex II Activity Buffer, (72582-25ML)
2 x 1 ml	10X Detergent, (72583-1ML)
500 µl	50X Succinate Solution, (72584-500UL)
250 µl	100X DCPIP, (72585-250UL)
60 µl	Ubiquinone 2, (72586-60UL)
5 ml	10X Blocking Solution, (72587-5ML)
6 ml	Phospholipids, (72588-6ML)
1 ea	Microplate, (72589-1EA)

* This kit contains sufficient materials for 96 measurements (one 96-well microplate).

Storage: Store the 20X Buffer, Complex II Activity Buffer, 10X Detergent, 10X Blocking Solution, and Microplate at 4°C. Store the 50X Succinate Solution, 100X DCPIP,
Ubiquinone 2, and Phospholipids at –80°C The Mitochondrial Complex II Activity Assay Kit contains sufficient materials for 96 measurements (one 96-well microplate).