Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together: -MAPmates™ that require a different assay buffer -Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9) -PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701) -More than 1 phospho-MAPmate™ for a single target (Akt, STAT3) -GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Millicell® Cell Culture Inserts
Optimized cell growth, attachment and differentiationMore
Optimized cell growth, attachment and differentiation Less
With Millicell inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell inserts make it possible to study both sides of the cell monolayer.
Millicell inserts are available for 24-, 12-, or 6-well plates. The inserts are easily prepared for SEM and TEM visualizing techniques, and they are compatible with cellular and/or fluorescent stains.
Insert Formats
Millicell Hanging Inserts
For co-culturing and permeability assays
Unique design allows easier basolateral access than other hanging inserts with less risk of contamination
Available in 5 pore sizes and 3 diameters, including a 1 µm pore size that is optically transparent for better visualization by microscopy
NEW: Available preloaded in 24-well receiver plates or as individual inserts in 3 diameters
Millicell Standing Inserts
Promotes excellent cell growth and provides an exceptional opportunity for cell studies
Available with Biopore (PTFE) membrane, MF-Millipore (mixed cellulose esters) membrane, and polycarbonate membrane
Millicell Organotypic Insert
For high cell viability and superior study of three dimensional explant structure
Lower height allows them to fit inside a standard petri dish
The Biopore™ (PTFE) membrane provides high viability—for as long as 40 days—and excellent trans-membrane oxygen transport
The membrane is optically clear and optimized for long-term organotypic explant maintenance
Membrane Types
Biopore Membrane (hydrophilic PTFE)
For low protein-binding, live cell viewing, and immunofluorescent applications
MF-Millipore™ Membrane (mixed cellulose esters)
For exceptional anatomical and functional polarization
Isopore™ Membrane (polycarbonate)
For growth of attachment-dependent cells without matrix
PET Membrane (polyethylene terephthalate)
For growth of attachment-dependent cells without matrix
With Millicell® inserts, adherent or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.
Propagation of human embryonic stem cells in a microporous membrane-based indirect co-culture system Kelsey Albert, Steven Sheridan, Louise Laurent, Igor Ulitsky, Ron Shamir, Jeanne Loring, & Raj R. Rao Biochemical and Biophysical Research Communications
2010
Astrocyte growth effects of vascular endothelial growth factor (VEGF) application to perinatal neocortical explants: Receptor mediation and signal transduction pathways Nina Mani, Alfia Khaibullina, Janette Krum and Jeffrey Rosenstein Experimental Neurology 192 (2005); 394-406
2005
Cell Culture
Subcellular localisation of recombinant a and g-synuclein Christian Specht, Cezar Tigaret, George Rast, Agnea Thalhammer, York Rudhard and Ralf Schoepfer Mol. Cell. Neurosci., 28 (2005); 326-334
2005
Cell Culture
Establishment of the organotypic model of amyotrophic lateral sclerosis from the SD rats' spinal cord Diao ZY, et. al,Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese. Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese.
2005
Cell Culture
Neural stem cells protect against glutamate-induced excitotoxicitiy and promote survival of injured motor neurons through the secretion of neurotrophic factors Jeronia Llado, Christine Haenggeli, Nicholas Maragakis, Evan Snyder and Jeffrey Rothstein Mol. Cell. Neurosci. , 27 (2004); 322-331
2004
Cell Culture
Development of an in vitro blood-brain barrier model-cytotoxicity of mercury and aluminum. Toimela, T et. al.,Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82. Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82.
2004
Morphological differentiation of bone marrow stromal cells into neuron-like cells after co-culture with hippocampal slice Abouelfetouh, Ayman, et al Brain Research (2004), Volume 1029, Issue 1 pp 114-119
2004
Cell Culture
Effect of sodium bicarbonate on extracellular pH, matrix accumulation, and morphology of cultured articular chondrocytes. Waldman SD, Couto DC, Omelon SJ, Kandel RA Tissue Engineering (2004), Nov-Dec;10(11-12):1633-40
2004
FGF-10 plays an essential role in the growth of the fetal prostate. Annemarie A. Donjacour, Axel A. Thomson and Gerald R. Cunha Developmental Biology 261 (1): 39-54
2003
Cell Culture
Changes in lymphokine receptor expression and fatty acid composition of phospholipids and triacylglycerols in rat adipocytes associated with lymph nodes following a transient immune challenge. J. D. Priddle, C. A. Mattacks, D. A. Sadler, H. A. MacQueen and C. M. Pond Cell Biology International 27 (1): 23-29
2003
Why do the electrodes need to be replaced every six months?
In general, electrodes have a 6 month lifetime if they are used continuously. The electrodes have a silver-silver chloride pellete that is depleted everytime it is used. It is the silver silver chloride pellete that creates the potential.
What voltage value can I expect for my cell type?
We DO NOT recommend using this function on the instrument. The Millicell-ERS will make voltage readings on only a few kinds of high-voltage generating cells (for example, it will not work on MDCK cells). We do have information on epithelial cells which typically have voltage values of 0-30mV.
What resistance value can I expect for my cell types?
Some resistance values have been published for various cell types. Resistance measurements will also depend on the type of cell culture (i.e. cell line, tissue or native cells). In general, the resistance range for epithelial cells is 10-20,000 ohms/cm2. An example of an established epithelial cell line with well defined electrical resistance is MDCK cells. MDCK cells displaying tight junctions show resistance of 5000 ohms/cm2.
What is the porosity of the Millicell Culture Plate Inserts?
The porosity of the Millicell Insert membranes are as follows: HATF - approx 80% CM - approx 80% PC and PCF - approx 10%
What is the length of the legs on the Millicell Insert unit?
The legs on the Millicell unit are approximately 1-2mm.
What is the thickness of the Millicell-PC and -PCF membranes?
The thickness of the PC and PCF membranes are 10 um.
What is the thickness of the Millicell-CM membrane?
The thickness of the Millicell-CM memnbrane is approximately 50 um.
What is the overall height of the Millicell-CM Organotypic insert?
The overall wall height including the feet is 5 mm.
What Volume of media should be used on the inside and outside of the 12 mm and 30mm Millicell cell culture insert devices?
Inside and outside liquid heights are critical and must be adjusted until equal. The suggested volumes below can vary due to plate well volume variations allowed by different cell culture plate manufacturers.
12 mm inserts: Inside: 0.4 mL Outside: 0.6 mL
Which ECM coating do we recommend for endothelial cells?
We recommend the Type 1 Rat Tail Collagen for most endothelial cells. It is recommended to be done with ethanol wetting and is a very cost efficient way to do ECM and seems to be well tolerated by most endothelial cells.
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