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PF024 MMP-9, Active, Human, Recombinant

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PF024
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PF024-5UG
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      Description
      OverviewRecombinant, human MMP-9 purified from transfected mammalian cells and activated with APMA. Active MMP-9 enzyme is APMA-free.
      Catalogue NumberPF024
      Brand Family Calbiochem®
      SynonymsMatrix Metalloproteinase 9, Gelatinase B, 83 kDa Gelatinase
      References
      ReferencesParsons, S.L., et al. 1997. Br. J. Surg. 84, 160.
      Backstrom, J.R., et al. 1996. J. Neuro. 16, 7910.
      Lim, G.P., et al. 1996. J. Neurochem. 67, 251.
      Xia, T., et al. 1996. Biochim. Biophys. Acta 1293, 259.
      Sang, Q.X., et al. 1995. Biochim. Biophys. Acta 1251, 99.
      Zempo, N., et al. 1994. J. Vasc. Surg. 20, 217.
      Birkedal-Hansen, H. 1993. J. Periodontol. 64, 484.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Jeffrey, J.J. 1991. Semin. Perinatol. 15, 118.
      Liotta, L.A., et al. 1991. Cell 64, 327.
      Harris, E. 1990. N. Engl. J. Med. 322, 1277.
      Product Information
      ActivityΔA₄₀₅/hour/µg protein ≥8.0 in standard thiopeptolide hydrolysis assays.
      EC number3.4.24.35
      FormLiquid
      FormulationIn 50 mM HEPES, 10 mM CaCl₂, 20% glycerol, 0.005% BRIJ®-35 Detergent, pH 7.5.
      PreservativeNone
      Quality LevelMQ200
      Applications
      Application ReferencesZymography Xia, T., et al. 1996. Biochim. Biophys. Acta 1293, 259. Kleiner, D.E. and Stetler-Stevenson W.G. 1994. Anal. Biochem. 218, 325. Heussen, C. and Dowdle, E.B. 1980. Anal. Biochem. 102, 196. Substrate cleavage assay Xia, T., et al. 1996. Biochim. Biophys. Acta 1293, 259.
      Biological Information
      Purity≥90% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot into siliconized vials and freeze (-70°C).
      Global Trade Item Number
      Catalogue Number GTIN
      PF024-5UG 07790788056438