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Merck

119114

Luciferase Inhibitor II

The Luciferase Inhibitor II, also referenced under CAS 10205-56-8, controls the biological activity of Luciferase.

Synonym(s):

Luciferase Inhibitor II, 4-(1,3-Benzothiazol-2-yl)-N,N-dimethylaniline

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About This Item

Empirical Formula (Hill Notation):
C15H14N2S
CAS Number:
10205-56-8
Molecular Weight:
254.35
MDL number:
MFCD00937700
UNSPSC Code:
12352200
NACRES:
NA.28

Key Documents

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Product Name

Luciferase Inhibitor II, The Luciferase Inhibitor II, also referenced under CAS 10205-56-8, controls the biological activity of Luciferase.

SMILES string

[s]1c2c(nc1c3ccc(cc3)N(C)C)cccc2

InChI

1S/C15H14N2S/c1-17(2)12-9-7-11(8-10-12)15-16-13-5-3-4-6-14(13)18-15/h3-10H,1-2H3

InChI key

HYKGLCSXVAAXNC-UHFFFAOYSA-N

assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

pale green

solubility

DMSO: 0.5 mg/mL
ethanol: 3 mg/mL

shipped in

ambient

storage temp.

2-8°C

Quality Level

100

Related Categories

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Disclaimer

Toxicity: Harmful (C)

General description

A 2-arylbenzothiazole compound that acts as an inhibitor against the ATP-dependent Photinus pyralis (lucPpy) and Photuris pennsylvanica (lucPpe) luciferase activity (IC50 = 0.2 and 0.32 µM, respectively, with 10 µM ATP, 10 µM luciferin, and 10 nM respective enzyme). Unlike Luciferase Inhibitor I (Cat. No. 119113), Luciferase Inhibitor II is competitive with respect to luciferin and exhibits reduced potency against lucPpe-based commercial luciferase-luciferin formulations with high luciferin concentration (IC50 = 10 µM against Promega′s Kinase-Glo; ineffective against Kinase-Glo Plus and Kinase-Glo Max; at a 3-fold formulation dilution and 6.77 µM ATP).

Legal Information

Marketed by Promega Corp. under the registered trademarks.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Auld, D.S., et al. 2009. J. Med. Chem.52, 1450.
Auld, D.S., et al. 2008. J. Med. Chem.51, 2372.

Packaging

Packaged under inert gas

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Alexandre Berthier et al.
STAR protocols, 2(3), 100658-100658 (2021-07-22)
Here, we describe an optimized protocol to identify specific nuclear receptor ligands. First, to rule out any compound interference with luciferase activity per se, we describe an in vitro assay assessing potential inhibition or activation of luciferase enzymatic activity. Second

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