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ABN88

Anti-phospho-NR1 (Ser896) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

glutamate receptor, ionotropic, N-methyl D-aspartate 1, glutamate [NMDA] receptor subunit zeta 1, N-methyl-D-aspartate receptor channel, subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, glutamate [NMDA] receptor subunit zeta-1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
rat
Application:
IHC, WB
Citations:
8
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biological source

rabbit

Quality Segment

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), human (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

NCBI accession no.

NP_058706

UniProt accession no.

P35439

shipped in

wet ice

target post-translational modification

phosphorylation (pSer896)

Gene Information

human ... GRIN1(2902)
mouse ... Grin1(14810)
rat ... Grin1(24408)

General description

NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.
~130kDa observed

Immunogen

Epitope: Phosphorylated Ser896
KLH-conjugated linear peptide corresponding to rat NR1 phosphorylated at Ser896.

Application

Detect phospho-NR1 (Ser896) using this Anti-phospho-NR1 (Ser896) Antibody validated for use in WB, IH(P).
Immunohistochemistry Analysis: 1:25 dilution from a previous lot detected NR1 in normal rat hippocampus tissue.

Biochem/physiol Actions

This antibody recognizes NR1 when phosphorylated at Ser896.

Analysis Note

Control
Lambda phosphatase untreated and treated rat microsomal brain tissue lysates
Evaluated by Western Blot in lambda phosphatase untreated and treated rat microsomal brain tissue lysates.

Western Blot Analysis: 1 µg/mL of this antibody detected NR1 on 10 µg of lambda phosphatase untreated and treated rat microsomal brain tissue lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 06-640

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Storage Class

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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