04-282
Anti-phospho-EGFR (Thr654) Antibody, clone 3F2
clone 3F2, Upstate®, from mouse
Synonym(s):
ERBB, ERBB1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
3F2, monoclonal
Species reactivity:
mouse, human, canine, rat
Application:
ELISA, IP, WB
Citations:
6
biological source
mouse
Quality Segment
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3F2, monoclonal
species reactivity
mouse, human, canine, rat
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pThr654)
Gene Information
dog ... Egfr(404306)
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)
General description
180 kDa
Immunogen
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 654 in human EGFR
Application
Detect phospho-EGFR (Thr654) using this Anti-phospho-EGFR (Thr654) Antibody, clone 3F2 validated for use in ELISA, IP & WB.
Biochem/physiol Actions
Recognizes phosphorylated EGFR on Threonine 654
Physical form
100 µg (0.1 mg/ml) of mouse monoclonal IgG1 in PBS containing 0.09% sodium azide, PEG, sucrose and 50% glycerol
Format: Purified
Analysis Note
Control
Includes PMA and pervanadate treated Hep2G cell lysate as a positive control
Includes PMA and pervanadate treated Hep2G cell lysate as a positive control
Routinely evaluated by immunoblot.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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