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MABS2229-25UG Anti-UGT1A9 Antibody, clone 14G11

Anti-UGT1A9, clone 14G11, Cat. No. MABS2229, is a mouse monoclonal antibody that detects UDP-glucuronosyltransferase 1-9 and is tested for use in Immunocytochemistry.

MABS2229-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2229-25UG
      DescriptionAnti-UGT1A9 Antibody, clone 14G11
      Alternate Names
      • UDP-glucuronosyltransferase 1-9
      • EC:2.4.1.17
      • UDPGT 1-9
      • UGT1*9
      • UGT1-09
      • UGT1.9:UDP-glucuronosyltransferase 1-I
      • UGT-1I
      • UGT1I
      • UDP-glucuronosyltransferase 1A9
      • lugP4
      Background InformationUDP-glucuronosyltransferase 1-9 (UniProt: O60656; also known as EC:2.4.1.17, UDPGT 1-9, UGT1*9, UGT1-09, UGT1.9, UDP-glucuronosyltransferase 1-I, UGT-1I, UGT1I, UDP-glucuronosyltransferase 1A9, lugP4) is encoded by the UGT1A9 (also known as GNT1, UGT1) gene (Gene ID: 54600) in human. UGT1A9 is a single-pass membrane glycoprotein that belongs to the UDP-glycosyltransferase family. UDP-Glucuronosyltransferases are a major group of phase II drug metabolizing enzymes and each enzyme in this family displays a unique substrate specificity and tissue-specific expression patterns. UDP-Glucuronosyltransferases mediate the transfer of glucuronic acid from UDP-glucuronic acid to various aglycone substrates making them more hydrophilic and readily excretable. UGT1A9 is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to generate the mature form. It is involved in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. Two isoforms of UGT1A9 have been described that are produced by alternative splicing. Both isoforms are expressed in liver and kidney. However, isoform 2 lacks the transferase activity and acts as a negative regulator of isoform 1. Clone 14G11 is shown to recognize both glycosylated and de-glycosylated forms of UGT1A9, however, the reactivity is shown to be higher in de-glycosylated form. (Ref.: Oda, S., et al. (2012). Drug Metab. Dispos. 40(8); 1620-1627).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-UGT1A9, clone 14G11, Cat. No. MABS2229, is a mouse monoclonal antibody that detects UDP-glucuronosyltransferase 1-9 and is tested for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 32 amino acids from the N-terminal half of human UDP-glucuronosyltransferase 1-9 (UDT1A9).
      EpitopeN-terminal half
      Clone14G11
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 14G11 is a mouse monoclonal antibody that detects UDP-glucuronosyltransferase 1-9 (UDT1A9). It targets an epitope within 32 amino acids from the N-terminal half.
      IsotypeIgG2a
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • UGT1A9
      • GNT1
      • UGT1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight59.94 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HepG2 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected UGT1A9 in HepG2 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2229-25UG 04061841878346