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07-225-I-25UG Anti-TrkB

Anti-TrkB, Cat. No. 07-225-I, is a rabbit polyclonal antibody that detects TrkB and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

07-225-I-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue Number07-225-I-25UG
      DescriptionAnti-TrkB
      Alternate Names
      • BDNF/NT-3 growth factors receptor
      • EC:2.7.10.1
      • GP145-TrkB
      • Trk-B
      • Neurotrophic tyrosine kinase receptor type 2
      • TrkB tyrosine kinase
      • Tropomyosin-related kinase B
      Background InformationBDNF/NT-3 growth factors receptor (UniProt: Q16620; also known as EC:2.7.10.1, GP145-TrkB, Trk-B, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B) is encoded by the NTRK2 (also known as TRKB) gene (Gene ID: 4915) in human. TrkB is a single-pass type I membrane protein that is internalized to endosomes upon ligand-binding. It is synthesized with a signal peptide (aa 1-31) that is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 32-430), a transmembrane domain (aa 431-454), and a cytoplasmic domain (aa 455-822). TrkB is a receptor tyrosine kinase that is involved in the development and maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. In the central nervous system, its expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophthalmic nerve, the vestibula, and multiple facial structures. Upon ligand-binding, it undergoes homodimerization, autophosphorylation, and activation. It can then recruit, phosphorylate and/or activate several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. TrkB is also reported to play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. Following binding of BDNF to TrkB receptor, Tyrosine 816 is phosphorylated, which allows the binding of phospholipase C gamma and activation of signaling cascade.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-TrkB, Cat. No. 07-225-I, is a rabbit polyclonal antibody that detects TrkB and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected TrkB in human hippocampus membrane preparations.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected TrkB in human cerebral cortex tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal half of human TrkB (BDNF/NT-3 growth factors receptor).
      EpitopeC-terminal half
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects TrkB (BDNF/NT-3 growth factors receptor). It targets an epitope within 18 amino acids from the C-terminal half, comprising mostly of the extracellular domain.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Monkey based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NTRK2
      • TRKB
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~82 and 142 kDa observed; 92.13 kDa calculated. TrkB can also undergo a high degree of glycosylation. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Human Brain tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TrkB in Human brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-225-I-25UG 04065265324199