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MABN2643-25UL Anti-RAPGEF1 (C3G) Antibody, clone 3F6

Anti-RAPGEF1 (C3G), clone 3F6 , Cat. No. MABN2643, is a mouse monoclonal antibody that detects RAPGEF1 and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

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MABN2643-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2643-25UL
      DescriptionAnti-RAPGEF1 (C3G) Antibody, clone 3F6
      Alternate Names
      • Rap guanine nucleotide exchange factor 1
      • CRK SH3-binding GNRP
      • Guanine nucleotide-releasing factor 2
      • Protein C3G
      Background InformationRap guanine nucleotide exchange factor 1 (UniProt: Q13905; also known as CRK SH3-binding GNRP, Guanine nucleotide-releasing factor 2, Protein C3G) is encoded by the RAPGEF1 (also known as GRF2) gene (Gene ID: 2889) in human. RAPGEF1 is a ubiquitously expressed protein that regulates multiple cellular activities and is shown to be essential for early embryonic development in mammals. It is involved in cell proliferation, transformation, cytoskeletal organization, and apoptosis. It is induced during skeletal muscle cell and neuronal cell differentiation. Higher expression of RAPGEF1 is observed in adult skeletal muscle and placenta and in fetal brain and heart. Its phosphorylation at tyrosine 504 enhances its activity as Rap guanine nucleotide exchange factor. It has functions that depend on its catalytic activity as well as protein interaction domain. It contains four SH3 binding motifs (aa 281-292; 451-462; 538-549; and 606-617). It binds to SH3 domain of CRK and GRB2 and transduces signals from CRK to activate RAS. It plays a role in the establishment of basal endothelial barrier function and has a role in nerve growth factor-induced sustained activation of Rap1 and neurite outgrowth. Four isoforms of RAPGEF1 have been described that are produced by alternative splicing. (Ref.: Begum, Z., et al. (2018). Mol. Biol. Rep. 45(6); 1809-1819; Sasi Kumar, K., et al. (2015). Biochim. Biophys. Acta. 1853(10); 2629-2639).
      References
      Product Information
      FormatPurified
      Control
      • MilliporeSigma offers a comprehensive range of trusted and highly cited primary and secondary antibodies for your research needs. Explore our 94
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      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-RAPGEF1 (C3G), clone 3F6 , Cat. No. MABN2643, is a mouse monoclonal antibody that detects RAPGEF1 and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesTested applications

      Immunocytochemistry Analysis: A representative lot detected RapGEF1 in Immunocytochemistry application (Begum, Z., et al. (2018). Mol Biol Rep. 45(6):1809-1819).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated RapGEF1 in Immunoprecipitation application (Begum, Z., et al. (2018). Mol Biol Rep. 45(6):1809-1819).

      Western Blotting Analysis: A representative lot detected RapGEF1 in Western Blotting application (Begum, Z., et al. (2018). Mol Biol Rep. 45(6):1809-1819; Sriram, D., et al. (2020). Sci Rep. 10(1):18838).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenGST-tagged recombinant protein fragment corresponding to the Crk binding region of human RAPGEF1 (aa 268-648).
      EpitopeInternal
      Clone3F6
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 3F6 is a mouse monoclonal antibody that detects Rap guanine nucleotide exchange factor 1 (RAPGEF1). It targets an epitope from the internal region.
      IsotypeIgG1κ
      Species Reactivity
      • Monkey
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Monkey, Mouse, Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RAPGEF1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~75 kDa observed for fusion protein of GFP with the Crk binding region (CBR) of RAPGEF1; 120.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cells transfected with GFP-Crk-binding region (CBR).

      Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected RAPGEF1 in HEK293 cells transfected with GFP- Crk-binding region (CBR), but not in cells without GFP-CBR transfection.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2643-25UL 04065269048282