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MABN2475-25UG Anti-NMDAR2B Antibody, clone 11D1

Anti-NMDAR2B, clone 11D1, Cat. No. MABN2475, is a highly specific mouse monoclonal antibody that targets Glutamate receptor ionotropic, NMDA 2B and has been tested for use in Western Blotting.

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MABN2475-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2475-25UG
      DescriptionAnti-NMDAR2B Antibody, clone 11D1
      Alternate Names
      • Glutamate receptor ionotropic NMDA 2B
      • GluN2B
      • Glutamate [NMDA] receptor subunit epsilon-2
      • N-methyl D-aspartate receptor subtype 2B
      • NR2B
      • N-methyl-D-aspartate receptor subunit 3
      • NR3
      • hNR3
      Background InformationGlutamate receptor ionotropic, NMDA2B (UniProt: Q13224; also known GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B, N-methyl-D-aspartate receptor subunit 3, NR3, hNR3) is encoded by the GRIN2B (also known as NMDAR2B) gene (Gene ID: 2904) in human. NMDA receptor complexes are ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. They are involved in long-term potentiation and neuronal plasticity and regulate the number of nerve cells during development. However, over-stimulation NMDA receptors can lead to neuronal degeneration. NMDAR2B is a heterotetrameric, multi-pass membrane protein that is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off in the mature form. The heterotetrameric channel is composed of two zeta subunits and two epsilon subunits, but can also form channels that contain at least one zeta subunit and at least one epsilon subunit. Activation of NMDA 2B channel requires binding of glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by magnesium ions. NMDA 2B is reported to be phosphorylated at Ser1303 by DAPK1 that enhances its channel activity allowing injurious Ca2+ influx that results in neuronal death. Mutations in NMDAR2B have been linked to early infantile epileptic encephalopathy that is characterized by refractory seizures and neurodevelopmental impairment. Some mutations are also known to cause mental retardation with or without seizures.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-NMDAR2B, clone 11D1, Cat. No. MABN2475, is a highly specific mouse monoclonal antibody that targets Glutamate receptor ionotropic, NMDA 2B and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected NMDAR2B in Western Blotting applications (Lu, W., et. al. (2017). Science. 355(6331)).

      Western Blotting Analysis: 4 µg/mL from a representative lot detected NMDAR2B in mouse brain tissue lysate.
      Biological Information
      ImmunogenFull length purified human Glutamate receptor ionotropic, NMDA 2B (NMDAR2B).
      Clone11D1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 11D1 is a mouse monoclonal antibody that specifically detects NMDAR2B.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GRIN2B
      • NMDAR2B
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight166 kDa observed; 166.37 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

      Western Blotting Analysis: 4 µg/mL of this antibody detected NMDAR2B in rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2475-25UG 04054839577208

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