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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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This Anti-IGF-IRα subunit Antibody, clone 1B1 is validated for use in Flow Cytometry, Immunocytochemistry, Western Blotting for the detection of IGF-IRα subunit.
More>>This Anti-IGF-IRα subunit Antibody, clone 1B1 is validated for use in Flow Cytometry, Immunocytochemistry, Western Blotting for the detection of IGF-IRα subunit. Less<<
Anti-IGF-IRα subunit Antibody, clone 1B1 : Malzeme Güvenlik Bilgi Formu (MSDS) veya SDS, Analiz Sertifikası (COA) ve Kalite Uygunluk Sertifikası (COQ), dosyalar, broşürler ve diğer dokümanlar.
Insulin-like growth factor 1 receptor (EC:2.7.10.1; UniProt P08069; also known as CD221, IGF-I receptor, Insulin-like growth factor I receptor) is encoded by the IGF1R (also known as CD221, IGFIR, IGFR, JTK13) gene (Gene ID 3480) in human. The insulin-like growth factor 1 (IGF-1) receptor is a receptor tyrosine kinase activated by IGF-1 and IGF-2. IGF-1 receptor is initially produced as a precursor protein with a signal peptide sequence (aa 1-30). Post-translational cleavages produce one extracellular alpha (aa 31-736) chain and one transmembrane beta chain (aa 741 – 1367) linked together by a disulfide bond. Two sets of alpha-beta dimers then form the functional tetrameric receptor complex via a disulfide linkage between their alpha chain. Upon ligand binding, the alpha chains induce the tyrosine autophosphorylation of the beta chains, which in turn trigger cell type-specific downstream signaling cascade of events.
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
This Anti-IGF-IRα subunit Antibody, clone 1B1 is validated for use in Flow Cytometry, Immunocytochemistry, Western Blotting for the detection of IGF-IRα subunit.
Key Applications
Flow Cytometry
Immunocytochemistry
Western Blotting
Application Notes
Flow Cytometry Analysis: A representative lot detected IGF-I receptor on the surface of MCF-7 human breast cancer cells (Nakadate, Y., et al. (2014). Monoclon. Antib. Immunodiagn. Immunother. 33(2):126-128). Immunocytochemistry Analysis: A representative lot detected IGF-I receptor on the surface of MCF-7 human breast cancer cells (Nakadate, Y., et al. (2014). Monoclon. Antib. Immunodiagn. Immunother. 33(2):126-128). Western Blotting Analysis: A representative lot detected the extracellular alpha (aa 31-736) chain of IGF-1R in MCF-7 cell extracts (Nakadate, Y., et al. (2014). Monoclon. Antib. Immunodiagn. Immunother. 33(2):126-128).
Biological Information
Immunogen
Recombinant protein corresponding to the extracellular fragment of human IGF-IR precursor protein.
Epitope
IGF-IR extracellular alpha chain.
Clone
1B1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 1B1 detected a band corresponding to the size of the alpha subunit of IGF-1R by immunoblotting analysis of recombinant protein and MCF-7 cell extracts (Nakadate, Y., et al. (2014). Monoclon. Antib. Immunodiagn. Immunother. 33(2):126-128).
Flow Cytometry Analysis: 0.1 µg of this antibody detected IGF-IRα subunit on MCF7 cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number
GTIN
MABS1301
04055977314991
Documentation
Anti-IGF-IRα subunit Antibody, clone 1B1 Certificates of Analysis
