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MABS2234-25UG Anti-ICA 512/RESP18 Antibody, clone 1348-D29-1

Anti-ICA 512/RESP18, clone 1348-D29-1, Cat. No. MABS2234, is a mouse monoclonal antibody that detects ICA 512/RESP18 and is tested for use in Immunofluorescence and Western Blotting.

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MABS2234-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2234-25UG
      DescriptionAnti-ICA 512/RESP18 Antibody, clone 1348-D29-1
      Alternate Names
      • Receptor-type tyrosine-protein phosphatase-like N
      • R-PTP-N
      • Islet cell antigen 512
      • ICA 512
      • Islet cell autoantigen 3
      • PTP IA-2
      Background InformationReceptor-type tyrosine-protein phosphatase-like N (UniProt: P16849; also known as R-PTP-N, Islet cell antigen 512, ICA 512, Islet cell autoantigen 3, PTP IA-2) is encoded by the PTPRN (also known as ICA3, ICA512) gene (Gene ID: 5798) in human. ICA 512, a tyrosine phosphatase like intrinsic membrane protein, is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-34), which is subsequently cleaved off to produce the mature form that contains a lumenal domain (aa 35-575), a transmembrane domain (aa 576-600), and a cytoplasmic domain (aa 601-979). Its lumenal region contains the RESP18 homology domain that is localized in amino acids 35-131. The mature form can undergo cleavage to generate an N-terminal fragment (ICA 512-NTF), a transmembrane fragment (ICA 512-TMF), and a cleaved cytosolic fragment (ICA 512-CCF). ICA 512 expression is restricted to neuroendocrine cells and is detected in the pancreas, brain, and pituitary. It is involved in the biogenesis and turnover of insulin secretory granules (SGs) in pancreatic islet β-cells . It plays a role in insulin secretion in response to glucose. It is required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation. It plays a role in insulin secretion in response to glucose stimuli. It is also required for normal accumulation of secretory vesicles in hippocampus and pituitary. ICA 512-TMF is reported to regulate dynamics and exocytosis of insulin SGs. ICA 512-CCF is shown to translocate to the nucleus and promotes the expression of insulin and other granule-related genes. It enhances pancreatic b-cell proliferation by converging with signaling by STAT5B and STAT3. ICA 512-NTF containing the RESP18HD domain encodes for an intrinsically disordered region that acts as a condensing factor for the reversible aggregation of insulin and other β-cell proteins in a pH and zinc-regulated manner. (Ref.: Toledo, PL., et al. (2019). J. Biol. Chem. 294(21); 8564-8576; Mziaut, H., et al. (2008). Proc. Natl. Acad. Sci. USA. 105(2); 674-679; Trajkovski, M., et al. (2008). J. Biol. Chem. 283(48); 33719-33729).
      References
      Product Information
      FormatPurified
      Control
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      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-ICA 512/RESP18, clone 1348-D29-1, Cat. No. MABS2234, is a mouse monoclonal antibody that detects ICA 512/RESP18 and is tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesTested applications

      Immunofluorescence Analysis: A representative lot detected ICA 512/RESP18 in Immunofluorescence applications (Toledo, P.L., et al. (2019). J Biol Chem. 294(21):8564-8576).

      Western Blotting Analysis: A representative lot detected ICA 512/RESP18 in Western Blotting application (Toledo, P.L., et al. (2019). J Biol Chem. 294(21):8564-8576).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenGST-tagged recombinant protein fragment corresponding to the mature N-terminal fragment (without the signal peptide) of human ICA 512.
      EpitopeN-terminal half
      Clone1348-D29-1
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1348-D29-1 is a mouse monoclonal antibody that detects ICA 512/RESP18. It targets an epitope within the RESP18 homology domain within the N-terminal fragment.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PTPRN
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~110-140 kDa observed; 105.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from EndoC βH1 cells.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected ICA 512/RESP18 in EndoC βH1 cells, but not in cells subjected to RNAi knockdown.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2234-25UG 04065269049302