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MABC1748-100UL Anti-FIP200 Antibody, clone N1-8

Anti-FIP200, clone N1-8, Cat. No. MABC1748, is a mouse monoclonal antibody that detects FAK family kinase-interacting protein of 200 kDa (FIP200) and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

MABC1748-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1748-100UL
      DescriptionAnti-FIP200 Antibody, clone N1-8
      Alternate Names
      • RB1-inducible coiled-coil protein 1
      • FAK family kinase-interacting protein of 200 kDa
      Background InformationRB1-inducible coiled-coil protein 1 (UniProt: Q8TDY2; also known as FAK family kinase-interacting protein of 200 kDa, FIP200) is encoded by the RB1CC1 (also known as KIAA0203; RBICC) gene (Gene ID: 9821) in human. FIP200 plays an important role in several biological pathways including cell proliferation and autophagy. It regulates early and late events of autophagosome formation through direct interaction with Atg16L1. FIP200 expression has been shown in cytoplasm and in the nucleus. It serves as a positive regulator of the NF-kB and mTOR signaling. It also functions as a negative factor in FAK-Erk1/2 signaling pathway. It is reported to form a complex with p53 and/or hSNF5 and the large protein complex in mammalian cells and serves as a strong activator of retinoblastoma 1 (RB1), p16, and p21 promoters. The nuclear FIP200 is shown to enhance the RB1 pathway globally and behave as a tumor suppressor in some human malignancies. Its expression levels correlate well with those of RB1 in cancer cell lines and higher expression has been shown in human musculoskeletal and cultured osteosarcoma cells. Clone N1-8 is highly suited for immunohistochemistry analysis and it recognizes an epitope within amino acids 255-271. (Ref.: Hama, Y., et al. (2012). PLoS ONE 7(3); e32052).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-FIP200, clone N1-8, Cat. No. MABC1748, is a mouse monoclonal antibody that detects FAK family kinase-interacting protein of 200 kDa (FIP200) and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • ELISA
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesELISA Analysis: A representative lot detected FIP200 in ELISA applications (Hama, Y., et. al. (2012). PLoS One. 7(3):e32052).

      Immunohistochemistry Applications: A representative lot detected FIP200 in Immunohistochemistry applications (Hama, Y., et. al. (2012). PLoS One. 7(3):e32052).

      Western Blotting Analysis: A representative lot detected FIP200 in Western Blotting applications (Hama, Y., et. al. (2012). PLoS One. 7(3):e32052).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 247 amino acids from the N-terminal region of human FIP200 and a C-terminal polypeptide fragment of FIP200.
      EpitopeN-terminus
      CloneN1-8
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone N1-8 is a mouse monoclonal antibody that detects Focal adhesion kinase (FAK)-interacting protein of 200 kDa (FIP200). It targets an epitope within 17 amino acids from the N-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RB1CC1
      • KIAA0203
      • RBICC
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight183.09 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human breast cancer tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected FIP200 in human breast cancer tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1748-100UL 04061842667628

      Documentation

      Anti-FIP200 Antibody, clone N1-8 MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-FIP200 Antibody, clone N1-8 Certificates of Analysis

      TitleLot Number
      Anti-FIP200, clone N1-8 - Q3509822 Q3509822

      References

      Reference overviewPub Med ID
      Preparation of mouse monoclonal antibody for RB1CC1 and its clinical application
      Yusuke Hama 1 , Tokuhiro Chano, Takuma Inui, Kyoichi Matsumoto, Hidetoshi Okabe
      PLos One  7(3)  e32052  2012

      Show Abstract       		22396748 22396748