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MAB1233-I-100UL Anti-Estradiol (6-BSA) Antibody, clone 6E1

Anti-Estradiol (6-BSA), clone 6E1, Cat. No. MAB1233-I, is a mouse monoclonal antibody that detects Estradiol-6-BSA and is tested for use in ELISA and Immunocytochemistry.

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MAB1233-I-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB1233-I-100UL
      DescriptionAnti-Estradiol (6-BSA) Antibody, clone 6E1
      Alternate Names
      • 17 beta-estradiol
      • E2-6-BSA
      • Estradiol BSA-Conjugated
      • 17β-estradiol 6-O-carboxymethyloxime-BSA
      Background InformationEstradiol is a major female sex hormone that regulates estrous and menstrual cycle. It is also present in smaller quantities in males where it is an active metabolic product of testosterone. In females, it is mainly produced by the granulosa cells in the follicles of ovaries. Smaller amounts of estradiol are also produced by the adrenal glands. It acts as an agonist for the estrogen receptor (ER) and can bind to both ERa and ERb. It is synthesized from cholesterol where androstenedione is a key intermediate which is converted to estradiol by aromatase. It is metabolized via hydroxylation into catechol estrogens. In the liver it can undergo hydroxylation into 2-hydoxyestradiol by CYP1A2, CYP3A4, and CYP2C9. Estradiol is reported to directly act on the central nervous system to modulate energy balance and reduced levels of estradiol following ovarian insufficiency have been linked to menopause, hyperphagia, and weight gain. (Ref.: Gonzales-Garcia, I., et al. (2018). Cell Rep. 25(2): 413-423).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Estradiol (6-BSA), clone 6E1, Cat. No. MAB1233-I, is a mouse monoclonal antibody that detects Estradiol-6-BSA and is tested for use in ELISA and Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      • Activity Assay
      • ELISA
      Application NotesELISA Analysis: Various titrations (2-fold serial dilutions) starting at 100 ng/mL from a representative lot detected Estradiol (6-BSA).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenBSA-conjugate Estradiol (Estradiol-6-BSA).
      EpitopeUnknown
      Clone6E1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 9E1 is a mouse monoclonal antibody that detects BSA-conjugated Estradiol.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in OVCAR 3 cells.

      Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected Estradiol (6-BSA) in OVCAR 3 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1233-I-100UL 04065265944922