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AP1043 Anti-Caspase-1 Mouse mAb (14F468)

Anti-Caspase-1 Mouse mAb (14F468): Malzeme Güvenlik Bilgi Formu (MSDS) veya SDS, Analiz Sertifikası (COA) ve Kalite Uygunluk Sertifikası (COQ), dosyalar, broşürler ve diğer dokümanlar.

Synonyms: Anti-CASP1, Anti-Interleukin-1β convertase, Anti-IL-1BC, Anti-IL-1β-converting enzyme, Anti-ICE, Anti-Interleukin-1β-converting enzyme

AP1043
  
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      Overview

      Key Spec Table

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      Description
      Overview

      This product has been discontinued.





      Recognizes the ~45 kDa latent form of caspase-1 in HeLa and NIH3T3 cells. Also recognizes cleaved forms of caspase-1 that retain amino acids 371-390.
      Catalogue NumberAP1043
      Brand Family Calbiochem®
      SynonymsAnti-CASP1, Anti-Interleukin-1β convertase, Anti-IL-1BC, Anti-IL-1β-converting enzyme, Anti-ICE, Anti-Interleukin-1β-converting enzyme
      Application Data
      Detection of human and mouse caspase-1 by immunoblotting. Samples: Whole cell lysate from HeLa (lane A) and NIH3T3 cells (lane B). Primary antibody: Anti-Caspase-1 Mouse mAb (14F468) (Cat. No. AP1043) (0.1 µg/ml, HeLa lysate and 2 µg/ml, NIH3T3 lysate). Detection: chemiluminescence.

      Detection of human caspase-1 by staining paraffin sections. Sample: Colon carcinoma, formalin-fixed (panels A and B). Primary antibody: Anti-Caspase-1 Mouse mAb (14F468) (Cat. No. AP1043). Detection: DAB with hematoxylin counterstain.
      References
      ReferencesTatsuta, T., et al. 2000. J. Biol. Chem. 275, 14248.
      Li, P., et al. 1995. Cell 80, 401.
      Yuan, J., et al. 1993. Cell 75, 641.
      Cerretti, D.P., et al. 1992. Science 256, 97.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlHeLa and NIH 3T3 cells and colon carcinoma
      Preservative≤ 0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (0.5-2 µg/ml)
      Paraffin Sections (see comments)
      Application CommentsWill stain formalin-fixed, paraffin-embedded tissues, but requires titration for individual samples. The immunogen sequence is also conserved in rat caspase-1, but cross-reactivity has not been tested. Also recognizes cleaved forms of caspase-1 that retain amino acids 371-390. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (RKVRFSFEQPDGRAQMPTTE) corresponding to amino acids 371-390 of human caspase-1
      ImmunogenHuman
      Clone14F468
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      AP1043 0