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ABE1952 Anti-CRTC1

Anti-CRTC1 Antibody, Cat. No. ABE1952, is a highly specific rabbit polyclonal antibody that has been validated for use in Immunoprecipitation and Western Blotting.

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ABE1952
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE1952
      DescriptionAnti-CRTC1
      Alternate Names
      • CREB-regulated transcription coactivator 1
      • Mucoepidermoid carcinoma translocated protein 1
      • Transducer of regulated cAMP response element-binding protein 1
      • TORC-1
      • Transducer of CREB protein 1
      Background InformationCREB-regulated transcription coactivator 1 (UniProt: Q6UUV9; also known as Mucoepidermoid carcinoma translocated protein 1;Transducer of regulated cAMP response element-binding protein 1;TORC-1;Transducer of CREB protein 1) is encoded by the CRTC1 (also known as MECT1; TORC1; WAMTP1; KIAA0616) gene (Gene ID 23373) in human. CRTC1 is a 67.3 kDa protein that activates transcription through binding cAMP response element (CRE) sites on DNA. It is highly expressed in adult and fetal brain and is localized to specific regions such as the prefrontal cortex and cerebellum. Very low expression is observed in heart, spleen, lung, skeletal muscle, salivary gland, ovary, and kidney. Phosphorylation/dephosphorylation states of Ser151 are required for regulating transduction of CREB activity. It is mainly localized in the cytoplasm when phosphorylated by salt-induced kinase (SIK) or AMPK and when sequestered by 14-3-3 proteins. It is translocated to the nucleus upon dephosphorylation on Ser151. CRTC1 is shown to mediate early beta-amyloid-induced transcriptional and memory deficits and overexpression of CRTC1 is shown to rescue amyloid-induced transcriptional and cognitive deficits. CRTC1 is reported to ameliorate hippocampal-dependent learning and memory deficits in APPSw,Ind mice by enhancing the expression of a specific subset of CCTC1 target genes. A chromosomal aberration involving CRTC1 has been reported in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CRTC1 Antibody, Cat. No. ABE1952, is a highly specific rabbit polyclonal antibody that has been validated for use in Immunoprecipitation and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: A 1:2,000 dilution from a representative lot detected CRTC1 in 10 µg of rat hippocampus lysate.

      Immunoprecipitation Analysis: 4 µL from a representative lot immunoprecipitated exogenously expressed mouse CRTC1 from transfected HEK293 cells (Courtesy of Dr Carlos A. Saura, Institut de Neurociències, Universitat Autònoma de Barcelona, Spain).

      Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected exogenously expressed mouse and rat CRTC1 in transfected HEK293 cells, as well as endogenous CRTC1 in mouse and rat hippocampus tissue homogenates (Courtesy of Dr Carlos A. Saura, Institut de Neurociències, Universitat Autònoma de Barcelona, Spain).
      Biological Information
      ImmunogenGST-tagged recombinat 135-amino acid fragment from the N-terminal region of human CRTC1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityABE1952 detected a target band in lysates from mouse and rat CRTC1-transfected HEK293 cells.
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteMouse, Rat. Predicted to react with Human based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CRTC1
      • MECT1
      • TORC1
      • WAMTP1
      • KIAA0616
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~78 kDa observed. 67.3 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse hippocampus tissue lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected CRTC1 in 10 µg of mouse hippocampus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1952 04054839135255

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