04-135
Anti-Brachyury Antibody, clone 3E4.2
clone 3E4.2, Upstate®, from mouse
Synonym(s):
T brachyury (mouse) homolog, T brachyury-like, T protein, T, brachyury homolog (mouse), transcription factor T
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
3E4.2, monoclonal
Application:
WB
Citations:
11
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3E4.2, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
input
sample type mesenchymal stem cell(s)
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TBXT(6862)
General description
47 kDa
The Brachyury protein is a T-box transcription factor that is one of the earliest indicators of mesoderm formation during embryonic development. As the brachyury protein family is essential for the formation of the posterior body in all vertebrates, Brachyury has been shown to be expressed during gastrula and neurula stages, as well as in the developing notochord. Consequently, when mutations occur within the brachyury gene, severe vertebral malformations have been found to occur, as a direct result. In addition, current findings also indicate that the dysregulation of brachyury may also be linked to the formation of chordomas, which are malignant tumors that develop along the spine.
Immunogen
Epitope: C-terminus
Synthetic human brachyury peptide, a.a. 224-435.
Application
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells
Mesenchymal Stem Cells
This Anti-Brachyury Antibody, clone 3E4.2 is validated for use in WB for the detection of Brachyury.
Biochem/physiol Actions
Reactivity with other species has not been tested.
This monoclonal antibody recognizes the brachyury protein.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.
Preparation Note
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.
Analysis Note
Routinely evaluated by Western blot on NT2 cell lysates.
Western Blotting:
Recommended working dilutions are 1:1000.
Western Blotting:
Recommended working dilutions are 1:1000.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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