Millipore Sigma Vibrant Logo
 
Life Science Research
 

Magnetic Beads - Recombinant Proteins

 Request Information

Advantages of PureProteome™ Nickel Magnetic Beads:

  • Be efficient with high capacity nickel beads: bind 28 mg His-tagged protein/mL settled beads.
  • High affinity nickel beads give you peace of mind: proteins bind beads tightly in buffers containing EDTA
  • Achieve high purity and yields: low binding of untagged proteins yields highly pure His-tagged proteins
  • Faster: shorten your protocol by 15 to 30 minutes
  • Easier to use: clearly visible beads enable you to monitor each step.
As shown in the Coomassie-stained gel, Merck's nickel magnetic bead system can give you greater purity of protein than other magnetic beads, without compromising yield.

PureProteome™ beads vs agarose beads and other magnetic beads.
LEFT: Polyhistidine-tagged 24 kDa protein purified from 1 mL E. coli culture with PureProteome™ beads (lane 7) and non-Merck magnetic beads (lanes 3-6). Coomassie blue-stained SDS-PAGE gel also shows MW standards (lane 1) and starting lysate (lane 2).

RIGHT: Purification of 6X-His-tagged C-RP expressed in E. coli. 25 μL of settled beads (PureProteome™: 100 μL 20% slurry; Competitor G: 40 μL 50 % slurry) were washed in 10 mL binding buffer, then incubated with 500 μL E. coli lysate for 30 minutes at RT with end-over-end mixing. Beads were then washed three times with wash buffer containing 20 mM imidazole, then eluted with two fractions of 100 μL elution buffer containing either 250 mM imidazole (PureProteome™) or 500 mM imidazole (Competitor G). 10 μL of each sample was loaded.